基于天然抗菌肽结构及抗菌机制的研究，开发安全、有效的肽类抗生素药物，是解决微生物耐药性这一世界难题的科学前沿。MD-AMPs-17是课题组从微生物感染12h后构建的家蝇转录组数据库中挖掘到的高表达基因，其在Genebank上尚未有功能注释，是一未知功能基因。前期采用原核表达系统成功获得重组MD-AMPs-17蛋白，体外证实了对G+、G-及真菌均具有抗菌活性，抗白假丝酵母菌活性最强。本项目在此基础上，拟深入研究重组MD-AMPs-17蛋白的抗真菌活性、稳定性及分子结构，探讨其结构与功能的关系；采用分子生物学、圆二色谱、激光共聚焦显微镜、电镜、流式细胞仪及光谱分析等方法，通过观察其对真菌的胞外结构（细胞壁、细胞膜）、胞内核酸的损伤以及菌体黏附、菌丝态形成和被膜形成的干预作用及其分子机制，从细胞水平和分子水平深入探讨该重组蛋白对白假丝酵母菌的抗菌机制，为开发新型高效抗菌药物提供理论基础和实验依据。

The research about structure, function and antibacterial mechanism of natura antibacterial peptides , development of safe and effective peptide antibiotics to solve the microbial resistance, that have become a new focus of antibacterial peptide research. MD - AMPs - 17 is a highly expressed genes which mined from house transcriptome database. There has not functional annotation in the Genebank about the genes. It’s an unknown function genes. In previous studies, recombinant MD-AMPs-17 protein was successfully obtained by prokaryotic expression system. It showed antibacterial activity against Escherichia coli, Staphylococcus aureus and Candida albicans. The activity was strongest against Candida albicans. On this basis, this project intends to study the antifungal activity, stability and molecular structure of recombinant MD-AMPs-17, to explore the relationship between structure and function; The antifungal mechanism will be studied by molecular biology technology, circular dichroism spectra, laser confocal microscopy, electron microscope, flow cytometer, flow cytometry and spectral analysis, to observe the extracellular structure (cell wall, cell membrane) and intracellular nucleic acid damage, thalline adhesion, hyphal formation and biofilm formation. The antibacterial mechanism of the recombinant peptide against Candida albicans will be investigated at the cellular and molecular level. The purpose of this study is to provide theoretical basis and experimental basis for the development of new and highly effective antibacterial drugs.