皮肤受损后的快速愈合对人体至关重要，但创伤修复仍然是临床中让人比较头疼的问题，鉴于目前对皮肤损伤继发的难愈合性皮肤创伤尚无有效治疗方法，寻找新型促创面愈合药物迫在眉睫。作为天然活性多肽资源宝库的两栖动物皮肤具有出色的创伤修复能力，然而相关的活性多肽分子还知之甚少。项目组在前期研究工作基础中，通过多肽分离纯化，发现云南臭蛙皮肤分泌物中含有数目庞大的新型促创伤修复活性多肽分子群。本项目旨在通过蛋白质/多肽组学、基因组学结合药理学活性追踪手段，全面识别云南臭蛙皮肤中促创伤修复肽类分子群，测定其在细胞水平和动物水平上的促创伤修复活性，开展其结构与功能研究，在分子水平上阐明其促创伤修复的分子机制。项目的顺利实施有望极大的丰富我们对两栖动物皮肤促创伤修复活性多肽的认知，为我们理解两栖动物强大的创伤修复能力提供新的分子线索，同时提供大量具有自主知识产权的新型促创伤修复活性多肽分子。

Rapid and effective wound healing of damaged skin is crucial for human beings, however, wound healing continues to be a considerable challenge in clinical trials. Considering that no satisfactory healing methods currently exist for secondary chronic wounds, the development of medicine and treatment to promote wound healing is crucial. As a natural resource of bioactive peptides, amphibian skins possess excellent wound healing potency and potential; however, the related molecular basis remains poorly understood. Through our initial peptide separation procedures, highly diverse novel wound-healing promoting peptides (WHPPs) were found in the skin secretions of the amphibian Odorrana andersonii. This program intends to 1) identify novel WHPPs in Odorrana andersonii skin secretions by a combination of proteomic/peptidomic, genomic, and pharmacological methods; 2) test the wound-healing promoting activities of these WHPPs on cellular and animal levels; and 3) perform the research of structure-functional relationship of WHPPs and 4)elucidate the related molecular mechanisms. This program will greatly increase our knowledge on the information of WHPPs , provide novel clues to understand the molecular basis for the impressive wound healing potency of amphibian skins and great diversity of novel wound-healing promoting drug candidate