角质层蜡质与甘蓝型油菜抗（耐）逆性密切相关，但目前控制油菜角质层蜡质沉积的调控因子和作用机制还不清楚。我们利用GWAS从全基因组水平筛选到一个调控甘蓝型油菜角质层蜡质沉积的转录因子BnMYB46，亚细胞定位表明BnMYB46具有核定位功能，其转录水平与甘蓝型油菜叶片蜡质沉积相关；蜡质相关基因启动子分析表明含有MYB46特异性应答元件M46RE，可能被BnMYB46所识别。为了揭示BnMYB46在甘蓝型油菜蜡质沉积中的功能与作用机制，本项目拟通过上调和下调BnMYB46的表达，分析其在蜡质沉积中的作用；进而利用转录组分析明确其调控的靶基因，确定其与靶基因启动子的结合特性及其对靶基因的调控活性；进一步分离BnMYB46基因的启动子，筛选调控BnMYB46的转录因子，从而阐明BnMYB46的上下游调控网络及其在蜡质沉积中的作用和调控机制。研究结果将为提高油菜抗（耐）逆性提供理论依据和基因资源。

As the outmost surface of plants, cuticular wax is related to the adaptation of Brassica napus to biotic and abiotic stresses. However, the key genes regulating cuticular wax deposition in B. napus and their acting mechanism are still unclear. In our previous studies, using Genome-Wide Association Studies (GWAS), a candidate transcription factor regulating cuticular wax deposition in B. napus, BnMYB46, was obtained. Results of subcellular localization showed that BnMYB46 protein was located in the cell nucleus. The transcription level of BnMYB46 was related to cuticular wax deposition in B. napus. Furthermore, the promoter regions of many of the cuticular wax biosynthetic genes contain MYB46-binding cis-regulatory motif M46RE, suggesting that MYB46 directly regulates the genes involved in the biosynthesis of the cuticular wax compositions. In order to reveal the function and molecular mechanism of BnMYB46 as a central regulator for cuticular wax deposition in B. napus, we plan to 1) analyze the role of BnMYB46 in cuticular wax deposition by up regulating and down regulating the expression of BnMYB46 in plant; 2) identify the target genes of BnMYB46 by RNA-seq; 3) confirm that BnMYB46 directly binds the promoter of the target genes and activates the transcription of the cuticular wax biosynthetic genes. The promoter of BnMYB46 will be furtherly cloned and the upstream transcription factor regulating BnMYB46 will be screened. Our aim is to elucidate the transcriptional regulation network and the function of BnMYB46 that controls the cuticular wax in B. napus. The results of the research will provide theoretic foundation and gene resources in increasing the tolerance of B. napus to stresses through genetic manipulation methods.