生育中后期干旱胁迫导致小麦严重减产，生育前期适度的干旱锻炼可显著提高小麦对生育中后期严重干旱逆境的耐性。脱落酸（ABA）调控了干旱锻炼诱导的气孔运动，通过平衡蒸腾失水和光合气体交换，增强小麦对灌浆期干旱胁迫的耐性，但其具体的调控机制尚未明确。本项目拟通过气孔形态无损监测、荧光探针标记及酶学分析等手段，明确关键信号物质过氧化氢（H2O2）和一氧化氮（NO）在介导ABA调控干旱锻炼诱导气孔运动过程中的作用；并采用转录组学，蛋白质组学和生物信息学等手段综合系统分析H2O2和NO介导ABA调控干旱锻炼诱导气孔运动的信号调控网络，并综合生理生化结果，揭示在干旱锻炼诱导小麦对灌浆期干旱耐性形成过程中，H2O2和NO在介导ABA调控气孔运动中的生理机制。研究结果可为进一步阐明ABA在调控干旱锻炼诱导小麦耐旱性形成中的作用机制提供依据，并为通过干旱锻炼主动提高小麦抗逆性的调控技术研究提供理论与技术支持。

Drought stress is the largest constraint to wheat production, especially occurring during critical stages of wheat growth. Water-deficit priming is confirmed can improve tolerance to a later occurred severe drought stress. Both water deficit priming and abscisic acid (ABA) induce stomata behavior to balance transpiration and photosynthesis, thereafter to enhance plant tolerance to drought stress occurring during grain filling. However, the mechanisms of ABA in mediating signaling in stomatal movement by drought priming are largely unknown. In this proposal, the in situ detecting of stomata, fluorescent probe labeling techniques will be applied to analyze the role and mechanisms of hydrogen peroxide (H2O2) and nitric oxide (NO), which are critical signals in the pathway of ABA mediating stomatal behavior. Furthermore, transcriptome and proteome profiling will be employed to explore the downstream networks in H2O2- or/and NO-mediated ABA-induced stomatal movement during drought priming and drought stress. The mechanisms of ABA induced stomatal movement during drought priming induced drought tolerance are to be revealed. The results can help further unfold the role of ABA in the induced drought tolerance due to water deficit priming, and the underlying mechanisms.