大气臭氧层破坏导致地表UV-B辐射增强，严重影响作物生产。前期研究发现OsVHA-A基因突变水稻对UV-B辐射极其敏感，且响应UV-B辐射的类黄酮代谢受到抑制。本研究拟通过酵母双杂交技术，筛选与OsVHA-A蛋白存在互作的类黄酮代谢调控蛋白，并采用Pull-down和BiFC技术验证蛋白间的互作关系，同时，采用Western blotting和qRT-PCR技术，检测OsVHA-A与关键互作蛋白响应UV-B辐射胁迫的表达模式，并分析不同基因型水稻响应UV-B辐射的类黄酮代谢差异。此外，构建OsVHA-A基因超表达转基因水稻株系，检验OsVHA-A蛋白与类黄酮代谢互作蛋白在超表达植株中响应UV-B辐射的表达模式、以及类黄酮代谢变化，以期阐明OsVHA-A基因参与水稻类黄酮响应UV-B辐射胁迫代谢的作用机制，预期研究结果可为耐UV-B辐射水稻的栽培调控和分子改良提供理论与技术支持。

The enhanced UV-B radiation from atmospheric ozone layer depletion has seriously endangered the crop production. The previous studies have revealed that OsVHA-A mutant rice is extremely sensitive to UV-B radiation, and its flavonoids mechanism induced by UV-B radiation is also blocked. In order to study the involvement of OsVHA-A in flavonoids mechanism, the present study will screen the proteins that interacted with OsVHA-A, especially the functional proteins involved in flavonoids metabolism, in the tisues of rice plant responding to UV-B radiation throught the yeast two hybrid techonology, and then the interaction relationships between proteins will be tested by GST-pull down and BiFC techniques. Meanwhile, the western blotting and real-time PCR technologies will also be performed to investigate the expression profiles of OsVHA-A and its interacting proteins for rice plant responding to UV-B radiation. Subsequently, the differential flavonoids mechanism will be analysed in the two rice genotypes under the UV-B radiation stress. In addition, the interaction relationships of OsVHA-A with its interacting proteins, and the flavonoids component of various tisues will be tested for the OsVHA-A over-expression rice plant which suffered from supplementary UV-B radiation. The expected results will provide theoretical basis and technological support for enhancing rice UV-B tolerance by cultivation measures and molecular improvement.