孕妇外周血胎儿有核红细胞（FNRBCs）分析作为一种新型可重复非侵入性产前检测与诊断方法，临床上能够实现胎儿遗传疾病和先天畸形的早诊，同时可提供生长发育早期胚胎的细胞层面的生理信息用于研究。FNRBCs数量稀少，当前缺乏有效的分离、富集及分析的技术与设备。本项目计划利用有生物兼容性的可降解纳米材料制作形貌可控表面可修饰的三维纳米结构，集成可对于微量流体进行精确操控的微流控技术后实现FNRBCs高灵敏度高纯度的分离以及富集；在芯片中实现FNRBCs识别与计数，建立其数目与临床病理特征的关联；对于细胞本身开发基于芯片的荧光免疫、原位杂交以及相关基因分析测序的配套仪器。本项目一方面实现FNRBCs在无创性产前诊断的临床应用，进行对于胎儿的遗传及生理状况的全面诊断；另一方面为生命发育等研究的科研机构提供可靠采样设备及数据分析平台，使其在生命生长发育早期的细胞及其基因组研究上得以继续深入。

The detection and analysis of fetal nucleated red blood cells (FNRBCs) in maternal peripheral blood provides provides a novel repeatable non-invasive stratagem for prenatal diagnostics. It can realize the early diagnosis for fetal genetic diseases or birth defect clinically, as well as offer cellular and genome insights for embryological researches. However, the scarcity of FNRBCs and lack of techniques for isolation, enrichment and analysis limit its researches and clinical applications. Thus, this proposal desribes an stragatem for highly effective and efficient capture and release of FNRBCs, and furthur, automatic enumeration and genetic analysis can be implemented. 3D biodegradable nano-structure and microfluidic techniques are employed to relize the isolation, enrichment and release of FNRBCs, and also to reveal the biological and clinical insights based on on-chip fluorescent immunostaining, in sito hybridization and genetic sequencing. This proposal can not only can the clinical applications for non-invasive prenatal diagnostics, but also provide a reliable platform for sample collection and data analysis which facilitates the researches in embryo cells and their genomes.