动脉粥样硬化(AS)发病机制尚不明确,以往研究提示,肠道微生物及代谢物TMAO可能参与AS发生,对其进一步研究有可能产生新的突破.中医学认为痰瘀毒是致AS的主要病机.其发病特点与肠道菌群驱动TMA/FMO3/TMAO通路致AS具有极大相似性.故本课题将"痰瘀毒"理论与肠道菌群及代谢物相结合研究AS.并介入针灸治疗.希望为其防治提供新的思路.课题组前期研究提示,针灸能够有效干预缺血性心脏疾患及调整肠道菌群.因此.本课题在以往研究基础上.提出"针刺能够通过调整肠道菌群及代谢物TMAO抗AS”假说.以肠道菌群及TMAO为靶点,通过高通量测序等技术观察肠道菌群的变化,用RT-PCR和HPLC-MS/MS等方法观察TMA/FMO3/TMAO通路中TMA、FMO3、TMAO水平变化.探讨AS发生发展与肠道菌群及代谢物之间的关系,研究化痰祛瘀排毒针刺治法抗AS机制,为针灸防治AS提供新的理论及实验依据.

The pathogenesis of AS is not clearly identified yet.Previous studies suggest that intestinal microbiome and its metabolites TMAO are involved in the occurrence of AS, which may be a new breakthrough for the further research. Chinese medicine believes that phlegm,blood stasis and toxin is the main pathogenesis of AS,which has great similarity pathogenetic character with TMA / FMO3 / TMAO pathway driven by intestinal microflora induced AS. Therefore, we will do the research of acupuncture and moxibustion treatment of AS and hope to provide new ideas for its prevention and treatment by combining the theory of phlegm ,blood stasis and toxin with intestinal flora .The previous findings in the study conducted by this research group and suggest that acupuncture and moxibustion can play an effective role in relieving ischemic heart disease and can adjust the composition of intestinal flora. This study proposes the hypothesis that acupuncture can relieve AS by adjusting the intestinal flora and the metabolic product TMAO. Observing the changes of intestinal flora via high-throughput sequencing technology, and the levels of TMA,FMO3 and TMAO in TMA / FMO3 / TMAO pathway by RT-PCR and HPLC-MS/MS methods with intestinal flora and TMAO as targets. The objectives of the study are to investigate the relationship between the onset and progression of AS and intestinal microbiome and its metabolites, and to explore the mechanism of acupuncture in the treatment of AS by removing phlegm ,blood stasis and toxin ,which will provide a new theoretical and experimental evidence for applying acupuncture and moxibustion to the prevention and treatment of AS.