针对目前生物大分子稳定的纳米载体营养素生物利用途径与效率研究中普遍存在的体外模拟消化模型参数选择随意，胶束化转运与细胞直接摄取效率评价方法对胆盐胶束和纳米载体选择性差，以及由此带来的数据可靠性存疑和相关机制分析困难问题，本项目拟在建立动态实时调控的连续消化模型以及胶束化定向抑制与胶束、载体分速渗透相结合的营养素吸收模式评价方案的基础上，分析和确立生物大分子结构及消化特征与纳米载体结构稳定性的相关性；载体结构及表面特性与细胞直接摄取水平的相关性；油相组成与水解速率营养素胶束化效率相关性；以及载体界面大分子、油相及胆盐胶束与营养素的相容性对胶束化转运效率的影响机制。全面了解影响细胞直接摄取和胶束化转运两种生物利用途径之间竞争与共存关系的纳米载体物理化学和生物学特性，用于指导具有高生物利用率的营养素纳米载体的有效构建，并为进一步分析营养素生物利用途径对生物有效性的影响奠定基础。

In order to solve the problem based on the poor reliability of the data and the challenges in analyzing the involving potential mechanisms induced by random selection of parameters of in vitro digestion models in evaluating the biological routes and efficiencies of the nutraceutical encapsulated in nano-delivery systems by biopolymers, and the poor differences of the current micellization transport and directly cellular uptake of nanocarriers evaluation methods in distinguishing bile salt micelles and nanocarriers, in this project a dynamic in vitro nutrient absorption model to achieve real-time control and effective absorption system which evaluate orientation inhibition of micellization or differential transport of bile salts micelles and nanocarriers were established. On the basis of above scheme, the relationship between the structure and digestion properties of biopolymers and structural stability of carriers, the relationship between structure and surface properties of carriers and the level of cellular uptake, and the relationship among oils type, oils digestibility, and micellization efficiency of bioactive components would be analyzed. And, the mechanisms that the compatibility between interfacial biopolymers, oils phase, and bile salts micelles and nutrients influences on the efficiency of nutrients through micellization transport would also be evaluated. Comprehensive understanding of physicochemical and biological characteristics of nano-delivery systems which would affect the competition and coexistence relationship between cellular uptake and micellization uptake would be formed. Information obtained from all the work could provide guidance to the valid building of nanodelivery systems with high bioavailability of active components encapsulated, and lay the foundation of the research on the effect of biological utilization pathways on bioavailability of nutraceuticals.