柑橘黄酮（CF）具有多种生理功效，但其水溶性较差。增加水溶性以提高生物利用率、拓宽应用范围已成为CF利用产业急需解决的关键问题。表面活性剂等两亲性小分子包埋是黄酮主要增溶方法之一，但存在一定的缺陷。项目在前期研究基础上以不同结构的代表性CF（橙皮苷、橙皮素和川陈皮素）为研究对象，采用生物大分子衍生物-辛烯基琥珀酸淀粉酯（OSAS）为增溶辅料，构建不同的饱和CF/OSAS/水体系，研究OSAS增溶CF的构效关系、外界影响因素和动力学过程，建立溶解度与不同来源OSAS分子量、取代度、直链/支链比、浓度的关联模型，揭示CF在OSAS中的增溶规律；采用多种光谱、H1NMR、X射线衍射、差示扫描量热法、透射电镜等手段探讨CF 与OSAS 相互作用、CF 在OSAS聚集体中增溶位点与微环境，阐明OSAS介导的 CF 增溶机理。项目研究可为CF的增溶设计和调控提供理论基础，有助于促进在食品等行业中应用。

Citrus flavonoids (CF) own multiple biological functions but poor water solubility. To enhance their water solubility to increase bioavailability and broaden application scope has become crucial problem to be solved urgently in the CF utilization industry. Encapsulation by amphiphilic small molecules including surfactants is one of the methods to solubilize CF, but has certain defects. To solve this problem, the project, based on the previous work, will use octenyl succinic anhydride modified starch (OSAS), a kind of natural biological macromolecule derivative, as a solubilizing agent to improve the solubility of typical CF (i.e. hesperidin, hesperetin, and nobiletin) in water. Different systems of saturated CF/OSAS/water will be designed to investigate the relationship between the structural parameters of OSAS of different origin (i.e. molecule weight, degree of substitution, ratio of amylose to amylopectin) and CF solubility, as well as external influencing factors and dynamic process of solubilizing CF in water as affected by OSAS. Based on these, correlation models between the solubility of CF and concentration and structural parameters of OSAS will be established and the solubilization behavior of CF in water will be disclosed. The solubilization effect of OSAS on CF will be further investigated using multiple spectrometry, fluorecence probe, H1nuclear magnetic resonance (NMR), X-ray diffraction, differential scanning calorimetry, and transmission electron microscope to understand the interactions between OSAS and CF, the solubilization sites and micro-environment in these systems. The success of this project will provide foundamental new knowledge on how OSAS increase the solubility of CF in water and then may have potential to expand the applications of bioactive activities of CF in a wide range of industries including food sectors.