复凝聚是一种基于蛋白质和多糖之间静电相互作用的微囊化技术，具有包埋效率高、控释性强、安全性好等特点，但是利用该法制备的微胶囊必须进行交联以提高其对酸性环境的稳定性。美拉德反应能引起含-NH2化合物发生交联，因此可用于提高复凝聚微胶囊的稳定性。本项目以SPI-壳聚糖为模式复凝聚体系，以VE为模式芯材，比较三种不同美拉德反应模式对VE复凝聚微胶囊在pH4.0溶液中稳定性的影响，从SPI和壳聚糖活性基团含量、SDS-PAGE图谱、凝胶渗透色谱图谱、分子间相互作用及微观结构变化等方面系统探讨不同美拉德反应模式交联效果产生差异的机理，并研究具有特殊结构的复凝聚微胶囊通过美拉德反应发生交联的规律。本项目对于寻找安全可控的复凝聚微胶囊交联方法、推动复凝聚微囊化技术的实际应用具有重要科学意义。

Complex coacervation is a microencapsulation technique based on the electrostatic interaction between proteins and polysaccharides. This technique is highlighted by its high microencapsulation efficiency, excellent controlled-release possibilities and good safety. Nevertheless, the microcapsules obtained via complex coacervation must be cross-linked to improve their stability against acidic environment. The Maillard reaction can initiate cross-linking in amino compounds and hence can be used in coacervate microcapsules cross-linking. In this proposal, the SPI-chitosan combination is selected as the model coacervation system and VE is used as the model core to compare the effects of three Maillard reaction modes on the stability in pH4.0 solution of the resultant microcapsules and the mechanism for the different cross-linking performances between the three reaction modes are elucidated by determining the changes of the active group contents in SPI and chitosan, SDS-PAGE and GPC patters, intermolecular interaction, and microstructures. Meanwhile, the rules involved in the Maillard reaction-induced cross-linking of the microcapsules are investigated as well. This proposal is meaningful in exploring a safe and controllable coacervate microcapsule cross-linking method and promoting the practical application of the complex coacervation microencapsulation method.