体质决定病证易感性、类型及预后，其动物模型缺失成为制约中医体质研究的瓶颈之一。课题组基于前自然基金“猫吓鼠”恐伤肾，肾阳虚转录谱等研究，提出 “基于体质遗传的先天禀赋联合后天环境造模是创建肾阳虚体质动物模型的关键”。研究以SD大鼠为对象，经寒热趋向行为特征、阳虚体貌性状（参考人类阳虚体貌特征拟定大鼠阳虚体貌半定量化筛选标准）等筛选典型阳虚体质大鼠进行传代，对亲代孕鼠采用超声波驱鼠器施以恐吓，以“恐伤肾”之后天环境造模，强化肾阳虚体征繁育子一代，子一代再以同样方案繁育子二代，子二代再以同样方案繁育子三代，经三次传代（在课题规定一年期内最大传代数），用先天禀赋结合“恐伤肾”强化子代的肾阳虚体质偏颇，初步构建肾阳虚体质动物模型，推动中医体质学的实验研究和理论创新。

Constitution of TCM plays a vital role in susceptibility, transformation and prognosis of diseases. And the lack of constitution model has been one of fallbacks restricting the constitution research. Based on previous researches of "fear impairing kidney" and transcript profile of kidney-yang defiency, a hypothesis is illustrated that the key to establish an animal model of kidney-yang defiency is to combine natural endowment with acquired environment. The SD rats will be applied in this study. Only rats of typical yang defiency constitution are chosen for subculturing after an assessment of hot-cold approach behavior and yang-defiency appearance referring to human characteristics of yang-defiency constitution. The parental pregnant rats will be frightened by a ultrasonic rat repellent, thus creating an acquired environment for offspring modeling. The first filial generation is created with a enhancement of kidney-yang defiency characteristics. In the same way, the second and third filial generations will be created. After subcultured for three generations in one year maximally, the kidney-yang defiency bias is enhanced and a model of kidney-yang defiency constitution is established preliminarily. In this way, experimental study and theoretical innovation of constitution of TCM is promoted.

本研究在大样本SD大鼠种群中，采用冷热板示差法开展寒热趋向的行为学评价,筛选老龄阳虚大鼠，依据前期肾阳虚研究和阳虚体质动物模型预实验设计的《大鼠肾阳虚体质评价量表》对初筛纳入的阳虚大鼠开展体貌性状与活动度、疲劳耐受等体能评价，确定符合阳虚诊断的亲代老龄肾阳虚体质大鼠（肾阳虚衰），经自然交配, 孕鼠孕期给予(猫吓鼠)恐伤肾造模，(寒冷温度刺激)低温造模，(盐炙黄柏水灌胃)苦寒药造模等强化的阳虚造模条件干预，繁育子一代阳虚大鼠。研究初步论证SD大鼠种群中存在自发性阳虚倾向大鼠；先天禀赋（老龄阳虚）与阳虚环境（恐伤肾、低温）共同干预，可以构建肾阳虚大鼠模型；苦寒药造模对孕鼠存在重大损害，不应作为造模条件。研究论证了“基于体质遗传的先天禀赋联合后天环境造模是创建肾阳虚体质动物模型的关键”的假说，为中医体质动物模型研究提供了全新的思路和重要的实验支撑。