药对是单方和复方之间的桥梁，研究药对的作用机制能驭繁就简地揭示复方配伍内涵；体现系统生物学思想的代谢组学有望为中药药对的药效整体评价研究提供有效解决途径。因此，本项目拟采用液质联用、气质联用及核磁共振技术以及正交偏最小二乘法判别分析(OPLS-DA)结合统计全相关谱(STOCSY)的多变量模式识别代谢组学方法，研究豁痰通阳药对瓜蒌-薤白对大鼠心阳虚胸痹慢性心肌缺血模型代谢网络的干预。从能量代谢途径、物质传递途径及信息传导途径确认表征药效的生物标志物群及其动态变化规律，结合常规药理指标，从整体上分析阐释该药对的作用机制可能是促进脂肪酸氧化，调节脂类代谢以“豁痰”，调节糖酵解和三羧酸循环代谢以“通阳”，二者共同作用，相互影响以改善慢性心肌缺血，进而为中医药防治冠心病这一严重危害人类健康与生命的重大疾病提供科学依据，研究意义深远。

Herb couple is the bridge between simple prescription and compound prescription. To study the mechanism of herb couple can reveal the connotation of compound prescription briefly. The metabolomics reflecting the thought of systemic biology is hoped to provide the effective solving approach to the general evaluation of the herb couple efficacy. This subject will study on the intervention of Gualou-Xiebai activating yang by expectorant function on metabolic net of the chronic myocardial ischemia rats through gas chromatography-mass spectrometry(GC-MS/MS), nuclear magnetic resonance(NMR) technology and orthogonality partial least squares-discriminant analysis(OPLS-DA), applying multiple pattern recognition treated metabonomics method based on Statistical Total Correlation Spectroscopy(STOCSY). We hope to find the biological markers group characterized efficacy and its dynamic change law from the way of energy metabolism, mass transfer and information conduction, so that maybe we can find the connotation of expectorant function is to regulate lipid metabolism, and the connotation of activating yang is to adjust the glycolysis and tricarboxylic acid cycle(TCA), and furthermore we can provide scientific evidence of Chinese traditional medicine preventing and treating coronary heart disease(CHD) which seriously damages human being health and life.

本课题以瓜蒌-薤白药对为突破口，采取整体性研究策略，利用代谢组学技术初步揭示慢性心肌缺血的发病机制，以及瓜蒌-薤白对大鼠心阳虚胸痹慢性心肌缺血的作用机制：利用盐酸异丙肾上腺素注射液制造慢性心肌缺血模型，基于代谢组学研究方法发现，慢性心肌缺血扰乱了氨基酸代谢和蛋白质代谢途径，干预了脂类代谢，并影响了糖酵解过程，造成大鼠体内代谢紊乱。瓜蒌-薤白药对能有效治疗慢性心肌缺血大鼠，其作用机制可能与调节氨基酸代谢和蛋白质代谢，脂类代谢，以及糖酵解等途径有关。