根结线虫是最重要的植物病原之一，可危害三千多种植物，全球每年损失达五百亿美元，目前其已成为限制我国蔬菜安全生产关键因子，但仍缺乏安全有效防治措施，解析线虫效应子功能是获得防治新靶标及制订防治新策略的前提。本研究拟利用原位杂交、发育表达模式分析、Western杂交、免疫组织定位和病毒瞬时表达、酵母双杂交、Gst pull down和BiFC、植物转基因及转录组分析和RNAi技术等手段明确南方根结线虫MiPdi效应子的表达部位及在不同虫龄表达差异，获得MiPDI与拟南芥互作的蛋白，明确转MiPdi基因拟南芥其形态、基因表达差异及对线虫敏感性，利用RNAi和拟南芥缺失突变体研究其功能。明确其在植物体内严峻氧化还原环境下调控寄主PTI和ETI免疫反应的作用和方式及在帮助线虫建立寄生关系的功能。结果将为阐明根结线虫的致病机理提供重要数据，同时为寻找防治根结线虫的新靶标并制定新策略提供理论依据。

Root-knot nematodes is one kind of the most important plant pathogens. It can damage more than three thousand kinds of plants, and causes 50 billion US dollars losses annually in the world. Also the nematode has become a key limiting factor in the production of vegetables in China. However, there are no safe and effective control approaches for management of root knot nematode so far. Functional analysis of nematode effectors will contribute to new targets and strategies for its control. The study here will use the methods of in situ hybridization, developmental expression pattern analysis, Western blot, immunohistochemistry localization, transient expression via virus, yeast two-hybrid system, Gst pull down, BiFC, plant genetic transformation, transcriptional anlysis, RNAi, Arabidopsis thaliana mutant, etc. and explore the function of MiPDI, such as localization, expression at different stages, interaction protein in A. thaliana, and the morphology, gene expression differences and susceptibility to nematodes by overexpression of MiPDI in A. thaliana, how to manipulating plant immunity such as PTI and ETI and so on. These results will illustrate the function of the protein in the mechanism of regulating plant immunity in redox systems of plant after invasion into plant and helps in parasitism of the nematode, which findings will support to find a new target gene and then development of the new control strategy.