拟禾本科根结线虫(Meloidogyne graminicola)是我国重要的植物病原线虫之一，严重威胁水稻生产安全。利用诱导抗性防治植物病害是发展绿色防控技术的一种新的途径。硅可以作为一种激发子，诱导植物对真菌和线虫病害的抗性。本研究通过硅酸钾诱导水稻后接种拟禾本科根结线虫，评价拟禾本科根结线虫在水稻上的侵染与发育进度，检测根中过氧化氢和木质素的变化，显微观测根结中胼胝质的沉积以及巨细胞的发育情况，利用荧光定量PCR测定水杨酸、茉莉酸、乙烯、油菜素甾醇等代谢途径中防御相关基因的转录水平变化等，从生理生化和分子生物学角度证明硅诱导水稻对根结线虫抗性的机制。研究结果将为更深入地理解硅类化合物在诱导植物对线虫病害抗性中的作用机制、更好地发展线虫病害绿色防控技术等奠定基础。

Root knot nematode Meloidogyne graminicola has been one of the most important plant parastic nematodes in China, which will be a serious threat to rice production. Induced resistance has been a new way to control plant disease. Silicium can act as a trigger of plant resistance, leading to rapid counterattack against invasion and development of pathogen. To investigate the effect of silicium on defense related responses, rice (Oryzae sativa Nipponbare) was root-drenched with potassium silicate and then challenged with root-knot nematode M. graminicola, the effect of plant responses such as the production of hydrogen peroxide and lignin, callose deposition, giant cell and nematode development, and gene expression level in the Salicylic acid (SA), Jasmonic acid (JA), Ethylene (ET) and Brassinosteroids (BR) pathway was analyzed. Further cellular and molecular studies will be useful in disclosing mechanism of silicium-induced resistance and developing green nematode control strategies.