植物腺毛存在广泛，具有重要的生物学价值和应用价值，是研究植物细胞命运调控的模式系统。番茄VI型腺毛属于分泌型腺毛，对番茄抗虫防病毒具有重要贡献，目前没有相关基因报道。本项目一方面以前期鉴定的VI型腺毛控制基因h-VI为基础，研究由其介导的调控途径；另一方面以360份番茄重测序核心种质为材料，利用GWAS对番茄VI型腺毛调控基因进行定位，并辅以遗传分离群体进行验证并克隆主效基因。前期我们鉴定到5个VI型腺毛相关的位点，其中1个与前人结果一致，说明定位结果可靠。本项目进一步分析定位区域内的变异与VI型腺毛密度的相关性，筛选候选基因，进行转基因确定基因功能。在此基础上，采用酵母单/双杂交、ChIP-seq等方法筛选VI型腺毛控制基因的上游调控因子、互作蛋白以及下游靶基因，解析该型腺毛形成的分子机制。该研究结果对于全面揭示番茄VI型腺毛形成的调控途径以及丰富植物细胞命运调控的分子机理具有重要意义。

Plant trichomes are widely-existed structures, which have great biological and applicable value and serve as an excellent model to study plant cell fate determination. The type VI glandular-trichomes play important roles in decreasing the damage of insects and virus in tomato. The genes regulating this type of trichome formation are still unknown. On one hand, based on the identification of h-VI gene regulating the formation of type VI trichomes, we plan to characterize the regulatory pathway mediated by this gene; on the other hand, we plan to clone the main effect genes using 360 re-sequencing core cultivars of tomato as materials by the genome-wide association analysis (GWAS). We have mapped 5 type VI trichome related loci using GWAS. One of them are consistent with previous results, demonstrating that the result is very reliable. In this projest, we intend to determine the correlation between the sequence variation in the target regions and the density of the type VI trichomes in the re-sequencing materials and the segregating populations, and then determine the candidate genes. On this basis, combining with Y1H, Y2H and ChIP-seq, we will screen the regulators, interacting proteins and downstream targets, comprehensively illustrating the molecular mechanisms of the type VI trichome formation. It is well worthwhile to reveal the regulatory network of this type trichome formation and enrich the mechanisms of plant cell fate determination.