吲哚族硫苷与IAA分别在十字花科植物防御反应和生长发育过程中发挥着十分重要的作用。它们具有共同的前体物-吲哚三乙醛肟（IAOx），由CYP79B2/B3催化形成。因此，阐明CYP79B2/B3参与吲哚族硫苷与IAA代谢平衡的分子机制将为十字花科植物生长发育调控及其对害虫、病原菌防御提供理论依据。但关于吲哚族硫苷与IAA代谢平衡的分子机制研究尚未见报道。我们前期研究表明大白菜BrCYP79B2/B3基因在这两条代谢途径中的表达水平存在显著差异。本项目将通过研究BrCYP79B2/B3在大白菜吲哚族硫苷与IAA代谢中的功能分化，分析不同生长发育阶段及诱导或抑制吲哚族硫苷、IAA合成等条件下BrCYP79B2/B3及其调节基因的表达变化规律，构建调控网络，从而解析BrCYP79B2/B3通过功能分化及在关键调节基因的调控下，参与大白菜吲哚族硫苷与IAA代谢平衡的分子机制。

Indole glucosinolates and IAA play important roles in plant defense responses, growth and development in Brassicaceae, respectively. They are derived from the common metabolite, indole-3-acetaldoxime (IAOx) which is catalyozed by CYP79B2/B3. So, it is important to explore the molecular mechanism of CYP79B2/B3 related indole glucosinolates and IAA metabolism homeostasis. It will help us understand how to control the developmental process or enhance the insect or pathogen resistance for Brassica crops. However, to date, it has not been reported on the molecular mechanism of homeostasis between indole glucosinolates and IAA metabolism. Our former study had shown that the expression levels of BrCYP79B2/B3 were significantly different from each other in those two metabolic pathway. This project will study the functional differentiation of multiple BrCYP79B2/B3 genes in the biosynthetic pathways of indole glucosinolates and IAA. To explore the molecular mechanism of indole glucosinolates and IAA metabolism homeostasis, Chinese cabbage with treatments for enhancing or inhibiting indole glucosinolates or IAA biosynthesis, or from different developmental stages, will be analyzed for gene expression by RNA-Seq method. The expression pattern of BrCYP79B2/B3 and corresponding transcription factors are then analyzed. Based on the expression pattern, a gene regulatory networks will be constructed to analyze the the mechanism of indole glucosinolates and IAA metabolism homeostasis in relation with BrCYP79B2/B3 under control of regulators.