甘蓝自交不亲和性S受体激酶下游信号传导元件是ARC1的结论在2014年受到质疑和否定，这与我们课题组近年来的蛋白质双向电泳分析和转录组学研究的初步结果相吻合。本项目拟在这些初步结果的基础上，进一步利用生物质谱、酵母双杂交和Ni离子捕获剩余电泳等方法组合而成的新颖技术路线，对授粉引起的自交不亲和甘蓝柱头的差异表达蛋白和差异转录序列的编码基因进行克隆、体外表达和遗传转化分析，以期能克隆甘蓝S受体激酶下游未知信号传导元件的编码基因，为芸苔属自交不亲和性的分子研究提供新的内容。

The conclusion that ARC1 was the downstream signaling component of S receptor kinase in self-incompatibility pathway in Brassica, was under question and denied in 2014. This coincides with the preliminary results of two-dimensional electrophoresis of proteins and transcriptomic studies in our research group during recent years. To further understand the mechanism of self-incompatibility response and seek potential candidate proteins involved in downstream signaling components of S receptor kinase in self-incompatibility pathway, the proteomic, yeast two-hybrid system, in vitro expression, Ni ion trapping electrophoresis and genetic transformation approaches would be taken to analyze the differential expression protein and the differential coding gene sequence of transcription in self-incompatibility Brassica stigma. The project will make novel supplements for understanding the molecular mechanism of self-incompatibility in Brassica.