牡丹是我国的传统名花，也是重要的候选国花，但自然花期短且相对集中，限制了其产业化生产。采用特定品种进行花期调控，可实现牡丹的周年供花。本研究以前期研究中获得的对低温和赤霉素敏感、易于秋季成花的牡丹品种‘秋发一号’为材料，并以常规促成栽培品种‘洛阳红’为对照，针对低温与赤霉素这两大影响牡丹成花调控的重要因素，利用已有转录组数据结合RACE技术，发掘与低温及赤霉素调控相关的FLC、DELLA等成花关键基因，研究其在不同牡丹品种、不同成花阶段的网络调控差异；通过转化拟南芥，验证牡丹关键成花基因的功能；同时利用表观遗传学方法，分离与甲基化密切相关的基因组序列，研究FLC等成花基因的甲基化位点，明确DNA甲基化与牡丹成花的关系，最后综合上述研究结果及低温与赤霉素处理对牡丹生理生化及表型的影响，揭示秋发牡丹易于成花的分子调控机制。本研究可为提高牡丹促成栽培效率、培育四季开花牡丹品种提供理论依据。

Tree peony is not only one of the most important ornamental crops, but also the first candidate for Chinese national flower. However, industrial production is greatly limited due to its relative short and concentrated flowering period. The special cultivars with forcing culture can be achieved for the full year flowering. In this study, ‘Qiu Fa Yi Hao’, which is sensitive to low temperature and gibberellin (GA) in our previous study, will be used as the material to investigate molecular mechanism of flowering regulation of tree peony, and the traditional cultivar ‘Luo Yang Hong’ is used as control material. Key flowering genes (viz. FLC, DELLA etc.) which are easily affected by low temperature and GA will be isolated with rapid amplification of cDNA ends (RACE) method, and the genetic regulatory networks will be carried out in different cultivars on different developmental stages. Then the gene function will be identified by Arabidopsis genetic transformation. At the same time, the method of epigenetics is used to isolate genome sequence which is easily methylated, and determine the methylated sites of the flowering genes such as FLC. Finally, combined with the physiology changes and morphological characteristics with different low temperature and GA treatments, the molecular mechanism of flowering regulation for autumn-flowering tree peony will be deciphered, which will provide theoretical basis for improving forcing culture and the breeding of year-round cultivars.