结缕草属植物是一种优良的暖季型草坪草，具有抗逆性强和耐粗放管理等诸多优良特征，是盐碱地绿化的首选草种。通过快速高效的分子手段培育优质耐盐结缕草属新品种，是目前迫切需要解决的问题。但关于结缕草属耐盐分子机制的研究进展远不能满足这一需要。针对这一现状，本项目充分利用我单位拥有的丰富的结缕草属种质资源和耐盐杂交后代，从多个生长及生理反应指标对其进行耐盐鉴定，通过Slaf-seq技术对其基因组进行测序，获得基因组信息和大批量SNP标记，对群体结构、亲缘关系和连锁不平衡水平进行综合分析，最后采用GWAS技术对SNP标记与耐盐性进行关联分析，获得与耐盐性紧密关联的SNPs，并对其进行功能注释，对显著性最高的基因进行克隆和功能验证。本研究获得的显著关联的SNPs及其基因将进一步解释结缕草属耐盐分子机制，并用于育种。本研究获得的结缕草属基因组信息和大批量SNP标记将为结缕草属其它性状的研究奠定良好的基础。

Zoysia grass is a excellent warm season turfgrass with the character of tolerance to salt, drought and extensive management. It is the first selection to improve the saline soil by planting zoysiagrass. It is urgent to breed new zoysia cultivars with high turf quality and salt tolerant by molecular methods. However, the research progress of molecular mechenisms of slat tolerant on zoysiagrass is far behind this need. According to this situation, the abundant zoysia germplasm resources and hybrids with high salt tolerant in our institute will be used in this project. Salt tolerance will be identified by some symptoms of growth and physiology under salt stress. The genome will be sequenced by a recently developed Specific Length Amplified Fragment sequencing (SLAF-seq) method, and sufficient SNPs will be obtained. Group structure, relatedness and linkage disequilibrium will be analyzed based on the sufficient SNP markers. Finally, association analysis will be conducted between SNPs and salt tolerance. Significant SNPs related to salt tolerance through whole genome of zoysiagrass will be obtained. Functional annotation will be performed on the genes including the SNPs or near the SNPs. The genes with highest relationship to the salt tolerance will be cloned and its function will be verified. In this study, the molecular mechnisms of salt tolerance of zoysiagrass will be interpreted by the SNPs and related geges detected in this study. These SNPs and genes will be used in zoysia breeding and the study on the molecular machenisms of other important characters of zoysia.