菊花是全球重要的商品花卉。菊花的成花大多受到短日照环境的诱导，商品化周年生产主要靠光照的调节，耗能且费时，极大地增加了生产成本。因此，认知菊花的成花机理，培育光周期不敏感品种，始终是菊花品种创制的国际性研究热点。申请者近期的研究发现，核因子NFYBs家族中的多个成员在菊花中的过量表达或RNAi沉默能够显著影响转基因植株在非诱导开花条件下的开花时期。基于此，本研究拟进一步检测NFYB 重要成员对菊花成花的影响，解析所涉及的下游基因和miRNA调节网络，筛选和鉴定互作蛋白，从转录和转录后层面揭示核因子NFYB成员影响菊花成花的调节机制，为从进化的角度解析菊花开花的分子生物学补充新数据，进而为培育光周期不敏感的菊花新品种提供基因储备。

Chrysanthemum is an important commercial flower as cut flowers, potted flowers and landscape plant cultivated worldwide. As a typical kind of short day plant, Chrysanthemum’s commercialization anniversary production depends mainly on light regulation and control which is power-wasting and time-consuming and leads to a significant increase on cost of production. Therefore, mechanism research of floral induction and breeding of cultivar that is insensitive to the photoperiod are always critical subjects in Chrysanthemum molecular breeding. From our recent study, several members of NFYB gene family were found that could change the abiotic stress tolerance and influence flowering time of Chrysanthemum.In this study, we will continue to indentify the influence of NFYBs gene on the floral induction of Chrysanthemum, analyze the related upstream genes and miRNA regulating network, screen and determine interaction factors, reveal the regulatory mechanism of studied NFYBs gene that affects the floral induction of Chrysanthemum on the transcriptional and post transcriptional level. Our goal is to provide new data for Molecular Biology analysis of chrysanthemum blossom from an evolutionary perspective and to excavate the genes for further transgenic breeding of new Chrysanthemum cultivars with photoperiod insensitivity.