中文摘要
‘千瓣莲’是中国独特的荷花资源,属于荷花重瓣化的极端类型,其发生机制仍是空白。我们前期通过切片、电镜等方法对不同花型的荷花分析发现,和单瓣品种相比,‘千瓣莲’的雌雄蕊原基完全消失,形成若干个“花瓣增殖中心”。转录组芯片显示,千瓣的形成是由一个NeTP-1(Nelumbo THOUSAND PETALS-1)基因的表达异常引起。进一步分析表明,NeTP-1基因本身并未突变,其表达下调是由于启动子区域的突变造成。本研究在前期基础上,深入分析NeTP-1基因的表达模式及其受调控的方式,揭示‘千瓣莲’形成的分子机制,为将来培育荷花新的千瓣品种提供科学的理论依据。
英文摘要
‘Qianban’ lotus is a kind of unique lotus resources in China, which belongs to the extreme type of double flowers lotus, while its formation mechanism is remains unknown. Comparing with the single flower cultivar, the thousand-petalled cultivar produced neither stamen primordia nor carpel primordia but developed the tiny petals instead through paraffin section and SEM observation. Further, NeTP-1 (Nelumbo THOUSAND PETALS-1) gene was found to down-regulated expression and predict to influence the formation of ‘Qianban’ lotus by high-through transcriptome sequence analysis. Then the NeTP-1 gene was sequenced and a 28 bp deletion was found in the promoter region of the NeTP-1. Based on these, the expression pattern of NeTP-1 and relationship between NeTP-1 and ‘Qianban’ lotus formation will be revealed and discussed. And this regulation mechanism will provide scientific theory basis for creating new cultivars of thousand-petalled lotus.
