霍乱弧菌是一种重要的人类致病菌，本项目的主要目的是研究霍乱弧菌O抗原的多样性，O抗原多样性对于细菌的生存和致病性非常重要的。霍乱弧菌有200多种O血清群。我们将破译霍乱弧菌中尚没有被破译的30种O抗原基因簇的序列，解析未知的15种霍乱弧菌O抗原的多糖结构。这将使我们比较全面地掌握霍乱弧菌的O抗原结构和相应的基因簇序列， 将使我们对霍乱弧菌O抗原多样性的遗传和进化机理有更深刻的认识。

Vibrio cholerae is an important human pathogen. In this project, we will systematically investigate the O-antigen diversity of V. cholerae, which is essential for the survival and pathogenicity of bacteria.More than 200 O-serogroups have been identified in V. cholerae. In this project, we will sequenced and analyzed the O-antigen gene clusters of 30 V. cholerae O-antigens, and determined the chemical structures of 15 V. cholerae O-antigens. The finding will be useful for understanding the synthesis process of V. cholerae O-antigens, and provide novel insight for the genetic and evolution mechanisms for the generation of O-antigen diversity in V. cholerae.