蒙药材阿给，中文名冷蒿(Artemisia frigida Wild.)，为菊科蒿属多年生小半灌木，在内蒙古草原资源蕴藏量极为丰富。冷蒿是蒙古族常用止血消肿药物，全草入蒙药、入中药。随着内蒙古草原的开发利用，冷蒿野生资源遭到严重破坏，不同来源地药材疗效不稳定，影响冷蒿药效成分形成的因素研究仍较少，更未对影响冷蒿道地性形成的分子机制进行研究。本项目选取内蒙古不同地域的蒙药材冷蒿样地，通过对其生长生境的地理、气象和土壤等因子，与其共生的丛枝菌根真菌（AMF）以及遗传机制的研究，探寻影响冷蒿药材道地性形成的环境因子、生物因子（AM真菌），并探索内在的分子机制，确定影响冷蒿产量、有效成分的多种因素的主次序列，建立影响冷蒿道地性的综合评价数学模型，揭示冷蒿道地性形成的机理，为持续利用蒙药材冷蒿提供科学基础数据，为保护道地性冷蒿的种质资源提供科学决策的依据，为继承和发展蒙医药事业增添创新性内容。

Mongolian drug Agei, known as Artemisia frigida wild.,belongs to semishrub of Asteraceae Artemisia plants,which mainly distributed in Inner Mongolia grassland and was extremely rich in resource reserves. Artemisia frigida is a medicinal plant of Mongolia nationality, a traditional Chinese medicine which can stop the bleeding and reduce the swelling,so it attracted the attention of many Mongolian scholars. With the development and utilization of grassland in Inner Mongolia, Artemisia frigida wild resources have been seriously damaged,so medicinal efficacy of Artemisia frigida from different regions was very unstable,but research on the influence factors of Artemisia frigida pharmacodynamic components formation was still less, no more Artemisia frigida genuineness formation mechanism research. By taking Artemisia frigida in different areas of Inner Mongolia as researching target，the project will hope to reveal the mechanism of Artemisia frigida genuineness formation by research of geographical factor,meteorological factors,soil factors,symbiotic AM fungi of its growth habitats and inherent genetic level. This will ascertain the primary and secondary sequence of a variety of factors which influence the yield and effective components of Artemisia frigida,establish the influence of cold Artemisia authentic of comprehensive evaluation mathematical model of Artemisia frigida wild formation mechanism of genuineness, reveal Artemisia frigida ormation mechanism of genuineness.This project will provide basic scientific data for the sustainable use of Mongolian medicinal herb Artemisia frigida, provide scientific decision basis for germplasm resources protection the genuineness of Artemisia frigida and add innovative content for inheritance and development of Mongolian medicine cause.

本项目选取内蒙古的森林草原、典型草原、草甸草原、沙地及荒漠草原5种不同生境的蒙药材冷蒿，对其生长环境的地理因子、气象因子、土壤因子与冷蒿产量和品质相关性进行研究，研究显示不同生境冷蒿茎和叶中的总黄酮和总生物碱的含量存在着显著性的差异，叶中总黄酮和总生物碱显著高于茎中的含量(p<0.05)，且有荒漠草原茎和叶中总黄酮和总生物碱含量最高，沙地和典型草原次之，草甸草原和森林草原最低，其含量与纬度成负相关，而与温度成正相关的现象；也显示土壤pH、全氮、有机质、有效磷、速效钾以及铁、锰、锌对冷蒿化学成分积累的影响较大。确定影响冷蒿药材道地性形成的地理、气象和土壤主导因素，揭示了冷蒿道地性形成的可能机理。