寡毛类纤毛虫是海洋微型浮游生物的主要功能类群，对该类群的系统进化研究是理解海洋微型浮游生物类群适应性进化的重要途径。核糖体基因（rDNA）得出的寡毛类纤毛虫系统发生关系与形态发生学观点产生了诸多争议，不能解释砂壳目的几丁质壳等表型特征的演化。其主要原因是目前已知的寡毛类群的功能基因数据极少，绝大部分寡毛类不容易在实验室大量培养。本项目拟针对寡毛类纤毛虫的多个科级类群开展转录组测序，使用单细胞扩增技术克服不可培养类群mRNA获取的难题。通过高通量测序获得大量功能基因序列，对寡毛类纤毛虫这一重要海洋生态类群进行基因组系统学分析，同时重点选取壳的合成与形成有关的功能基因，分析相关蛋白基础上的系统进化历程，探讨其与其壳形态特征演化的耦合关系，为探讨原生动物形态演化、浮游生活的适应机制及对全球环境变化的响应等提供重要参考。

The planktonic oligotrich ciliates (Subclasses Oligotrichia and Choreotrichia)are key players in the micro-planctonic community. A comprehensive phylogeny to the oligotrichs is fundamentally important for our deeper understanding of the evolutionary and ecological adaptation of these planktonic protists. Nowerdays, phylogeny of oligotich families are mainly based on the rRNA genes, which however could not give consistant evolutionary trend to the morphology system, e.g. lorica for tintinnids. The phylogenetic relationships among oligotrichs are still poorly understood, and suffered from a lack of protein data. We propose to do a phylogenomic analysis for oligotrich ciliates, using quantity orthologues generated from transcriptome sequencing or single-cell amplification technique. Transcriptome will be sampled on family levels and used to deduce the evolution process among oligotrichs. Gene annotation will be carried to gain the main information of oligotrichs functionnal genes. Genes specifically related to lorica will also be used to deduce the adaptation evolution of oligotrichs. Overall, the outcome of this project will offer crucial references for the ecological and evolution studies to the marine planktonic protozoa.