竹子属于禾本科竹亚科(Bambusoideae)植物，被广泛的应用于医药、食品、建材、造纸等行业，其稀有的种子资源对竹林的可持续发展和避免生态灾难具有重要的意义。种子内生真菌与种子的萌发和幼苗的生长密切相关，并在防治病害、提高种子资源利用效率等方面发挥了重要的作用，但竹种子内生真菌的相关研究却少有人涉及。鉴于此，项目申请者在前期工作的基础上较系统的开展竹种子内生真菌的研究工作，从不同地区（广东、广西、贵州、海南）不同竹种（毛竹、麻竹、吊丝单）的种子中，分离培养内生真菌；通过形态观察和分子鉴定确定内生真菌的种类组成，利用真菌群落的结构变化反映内生真菌与生态环境、寄主种类的相关性；采用抑菌实验大规模快速筛选高生物活性的菌株，初步分析相关的有效成份。通过本项目的研究，收集大量的菌株，为进一步探讨竹种子与内生真菌的相互作用奠定基础，也为下一步研发新生防制剂和生物活性物质提供重要的资源。

Bamboos (Bambusoideae, Poaceae) have been used broadly for traditional medicine, food material, building element and paper pulp. However, there is a considerable ecological problem in most of bamboo speices, where sexual propagation plays a vital part in the sustainable production of bamboos, but the seeds are uncommon and have a low germination. ..The seed-associated fungal endophytes were usually implicated in assisting seeds in germination of seed pods and some fungal endophytes had serious negative effects on the seed survival in tissue culture. These seeds colonized by field and storage fungi could be a source of potential pathogens, which might pose problems in nurseries. Furthermore, many endophytic fungi are capable of synthesizing bioactive compounds which can be used by the plants for defense against pathogenic fungi and bacteria, and producing a wide range of novel metabolites of pharmaceutical and agricultural importance...Our project is for the first time to investigate the diversity of endophytic fungi associated with bamboo seeds comprehensively, and screen the antimicrobial activity of culturable strains. . In the 3-year plan, amount of fungal endophytes would be isolated from three types of bamboo (Phyllostachys edulis, Dendrocalamus latiflorus Munro, and Dendrocalamopsis vario-striata (W. T. Lin) Keng f.) seeds, at different locations (Guangdong, Guangxi, Guizhou, Hainan). These strains would be categorized into different morphotypes according to culturable characteristics and their taxonomic position would be validated by molecular identification. The diversities of the fungal community structures would be compared among three species of bamboos at the same location, or among different locations by the same species. Furthermore, inhibitory effects against clinical pathogens and phytopathogens would be screened globally and the candidate isolates should display high and broad-spectrum bioactivities by the agar diffusion method. The effective agents extracted from these represented strains under submerged fermentation, would be analyzed preliminarily and checked again for the antimicrobiol activities. .After the future project, a good deal of fungal strains would be collected from bamboo seeds, which could improve to understand the interaction between bamboo seeds and endophytic fungi, and be anticipated as a potential source of novel plant defense activators and bioactive compounds.