申请人从事环境微生物学研究10年，一直潜心于重要难降解有机污染物分解代谢的机理研究。研究成果主要以第一作者在遗传学领域PLoS. Genet.、微生物学领域MM（2篇）、AEM、JB、生化领域JBC等杂志上发表，总计发表SCI论文48篇(第一/通讯作者15篇)。文章他引超过400次（如被PNAS，EM等杂志引用，并被英国皇家学会院士Timmis教授编辑到著作中）。申请人首次全面系统的阐明困惑科学家近60年的假单胞菌分解代谢尼古丁吡咯途径的分子机理，如克隆15个尼古丁代谢关键新基因；提出新的HTH motif的蛋白结合DNA的调控机制；获得3个蛋白晶体结构，完成降解菌株的全基因组和比较蛋白组测序工作。拟在此基础上深入开展恶臭假单胞菌B6-2分解代谢二噁英类化合物如二苯并呋喃等分子机理研究，本项目的研究将会丰富微生物分解代谢环境有毒污染物基础理论、增加环境有毒物微生物降解多样性的认识。

The applicant engaged in environmental microbiology research for 10 years, and his research has been focused on the microbial catabolism of important organic pollutants. He has published a total of 48 papers, (15 as the first/corresponding author), in PLoS Genet of genetic field, MM (2), AEM, JB of microbiology field, and JBC of biochemistry field. The articles in these elite journals got more than 400 citations, such as in PNAS, EM and other peer-reviewed journals. Professor Timmis, who is the fellow of the Royal Society in UK, edited some of the results in his book. The molecular mechanism of nicotine degradation by Pseudomonas, which puzzled scientists for nearly 60 years, was comprehensively clarified for the first time in the world. Fifteen new key genes involved in nicotine degradation were cloned and identified. A new regulatory mechanism of HTH motif in DNA binding proteins was proposed. Three protein crystal structures were obtained. The genome sequencing and comparative proteomics of the degrading strain were completed. On this basis, the molecular catabolism of dioxin-like compounds, such as dibenzofuran, by Pseudomonas putida strain B6-2 will be studied. The research results in this project will enrich the basic theory in microbial catabolism of toxic environmental pollutants, and increase the diversity of microbial degradation of these poisons.

海上油气勘探与运输发展迅猛，由此带来的溢油风险也将长期处于高位。海底沉积物中氮循环关键环节微生物种群的多样性、丰度、演替规律及其长期响应是评价石油污染对近海生态功能影响的重要指标。本研究以大连湾溢油污染沉积环境为主要研究对象，结合原位连续跟踪采集分析与微宇宙模拟实验，应用宏转录组、克隆文库、实时荧光定量 PCR、硝化活性测定等现代生物学技术，结合石油组分、沉积物理化指标特征，耦合有机污染物代谢机制和关键微生物分析，探究了溢油污染后长时间序列微生物群落结构演替的一般特征，取样深度和pH是剖面微生物群落结构的最大影响因子。岸滩微生物群落演替规律与岸滩溢油组分变化耦合，短时间序列，岸滩潮上带溢油降解迅速，52 d内饱和烃和芳香烃基本降解完全，芳烃萘系物出现明显降解，对比短期自然修复，溢油两年后，芳烃的降解依然非常少，暗示大量残油沉积并不断向深层渗漏。自然修复过程中参与氮循环的各关键基因中，一些低氧条件下的氮转化过程在溢油和非溢油环境中相差不大。对于需氧反应，溢油促进固氮作用的发生，抑制硝化作用相关基因的活性。氨氧化过程受到溢油影响较大，沉积物中AOA生物量相较于AOB占据更大优势。而且，首次研究蓝藻对不同浓度原油的响应机制，探究蓝藻群落结构变化，并验证其爆发机理。