申请者主要从事有机污染物降解菌的代谢机理与污染环境微生物生态研究。在四个方面取得突出成果：1）获得有机污染物高效降解菌株50余株；2）首次从基础代谢网络调节角度阐明降解菌株响应有机污染胁迫其基础代谢物的演替规律和调控机制；3）成功将部分菌株应用于有机污染环境生物强化修复；4）系统研究了多个污染环境微生物群落结构变化与自然修复进程的关系，揭示墨西哥湾深海溢油污染水体中烃类降解菌群随石油污染进行着快速动态调适过程。目前已发表SCI论文44篇，累计引用676次；近五年以第一或通讯作者发表SCI论文17篇，包括ISME J、Appl Environ Microb、Environ Sci Technol等，其中IF>3的论文13篇，单篇最高IF5y=8.927，被国际同行正面引用246次。拟开展近海沉积物微生物群落结构和功能对溢油的生态响应研究。

The applicant mainly focuses on the metabolism mechanisms of organic pollutants by microorganisms and microbial ecology of polluted environment. She demonstrated her achievements in the following four research areas: 1) Isolated more than 50 bacterial strains capable of degrading various organic pollutants; 2) Elucidated the metabolic profiling response of degradation strains to the exposure of specific pollutants, revealing the regulation mechanisms at basal metabolism level of the strains during pollutant degradation, which will provide new ideas and evidence for the adaptive evolution of the degradation strains to polluted environment; 3) Successfully applied the strain Pseudomonas sp. HF-1 in the bioremediation of tobacco wastewater and tobacco wastes contaminated soil, and the mixed culture of Rhodococcus sp. strain YYL and two bacilli for treating THF wastewater; 4) Demonstrated the relationship between functional genes, microbial functional groups and ecosystem functions in the organics polluted environment. The microbial community functional composition and structure were dramatically altered in a deep-sea oil plume resulting from the Deepwater Horizon oil spill in the Gulf of Mexico, suggesting that the indigenous marine microbial communities could play a significant role in biodegradation of oil spills in deep-sea environments. Forty-four SCI articles were published including one co-authored Science paper. The total citation is 676 times. Seventeen SCI articles were published within 5 years as the first/corresponding author including ISME J, Appl Environ Microb, Environ Sci Technol, and Water Res, among them, 13 articles with impact factor (IF) > 3 and the highest IF5y=8.927. The first/corresponding articles were cited by other SCI papers for 246 times. The proposed research will focus on exploring the microbial community structure and their functions in response to oil pollution in marine sediments.

海上油气勘探与运输发展迅猛，由此带来的溢油风险也将长期处于高位。海底沉积物中氮循环关键环节微生物种群的多样性、丰度、演替规律及其长期响应是评价石油污染对近海生态功能影响的重要指标。本研究以大连湾溢油污染沉积环境为主要研究对象，结合原位连续跟踪采集分析与微宇宙模拟实验，应用宏转录组、克隆文库、实时荧光定量 PCR、硝化活性测定等现代生物学技术，结合石油组分、沉积物理化指标特征，耦合有机污染物代谢机制和关键微生物分析，探究了溢油污染后长时间序列微生物群落结构演替的一般特征，取样深度和pH是剖面微生物群落结构的最大影响因子。岸滩微生物群落演替规律与岸滩溢油组分变化耦合，短时间序列，岸滩潮上带溢油降解迅速，52 d内饱和烃和芳香烃基本降解完全，芳烃萘系物出现明显降解，对比短期自然修复，溢油两年后，芳烃的降解依然非常少，暗示大量残油沉积并不断向深层渗漏。自然修复过程中参与氮循环的各关键基因中，一些低氧条件下的氮转化过程在溢油和非溢油环境中相差不大。对于需氧反应，溢油促进固氮作用的发生，抑制硝化作用相关基因的活性。氨氧化过程受到溢油影响较大，沉积物中AOA生物量相较于AOB占据更大优势。而且，首次研究蓝藻对不同浓度原油的响应机制，探究蓝藻群落结构变化，并验证其爆发机理。