中文摘要
申请人研究领域是水稻功能基因组学和生殖发育生物学。围绕水稻功能基因组研究,建立和完善了品种中花11号的大型突变体库,提出了T-DNA偏爱插入在长度较大染色体及编码基因调控区的观点,为我国及世界水稻功能基因组研究发挥了重要作用。基于突变体资源,以水稻育性和抽穗期两个农艺性状遗传改良为目标,鉴定了绒毡层细胞程序性死亡的关键基因API5,表明花药壁绒毡层降解的时机和程度决定了花粉的育性;系统研究了减数分裂过程中同源染色体配对、重组和修复的分子作用过程,阐明了PAIR3和RPA家族基因在水稻雄配子体发生与发育中的功能;通过研究水稻成花转换的关键基因及其相互调控网络,发现禾本科植物存在其特有的成花调控路径。近五年来在Plant Cell、Plant Journal、Plant Physiology等国际主流杂志发表13篇通讯作者论文,通讯作者论文被SCI他引140次,获授权专利10项。
英文摘要
The applicant’s research areas are focused on the functional genomics and reproductive developmental biology in rice. A large T-DNA insertion library was generated using Zhonghua 11 variety, which plays an important role on the study of rice functional genomics in the world. Flanking sequence analysis revealed that T-DNA insertions were biased towards large chromosomes and regulatory regions of the genes. Base on these mutants, the applicant aims at the rice reproductive developmental process of male sterility and heading date as genetic improvement traits. A novel gene API5 regulating programmed cell death during tapetum degeneration was identified and characterized, suggesting that the timing and extent of tapetum degeneration in postmeiotic anthers is critical for the normal fertility in rice. Furthermore, the molecular mechanisms of chromosome pairing, synapsis and homologous recombination during meiosis have been investigated systematically. Those work uncovered many novel components, such as PAIR3 and RPA gene family, which are required for microgametogenesis in rice. By studying some novel genes involved in flowering transition in rice, it was proposed that a distinct flowering pathway maybe present in crops. In the last five years, as a corresponding author, the applicant has published 13 papers in prestigious journals, such as Plant Cell, Plant Journal, and Plant Physiology. Those papers have been cited for 140 times. The applicant obtained 10 authorized patents.
