本项目拟研制小动物三维在体神经回路成像设备，该成像设备以双光子激发成像模态为核心，有机融合光片照明显微成像模态，从细胞分子、结构图谱和功能回路多个层面系统全面地提供生物体的神经回路信息。围绕小动物三维在体神经回路成像设备研制这一核心目标，将会涉及到成像设备、图像算法、软件平台、验证评价以及生物医学应用等多方面研究。从生物体在体神经回路深层和快速的成像要求出发，研制有机融合多光子深层激发成像模态和光片照明快速扫描显微成像模态于一体的小动物三维在体神经回路成像设备，研发适用于快速动态神经回路成像的影像信息处理与分析平台，建立小动物三维在体神经回路成像设备的医学生物验证评价体系，开展小动物预临床生物医学应用研究，为小动物脑疾病模型在体神经回路的机理研究提供成像方法和工具。

This project is focused on developing in vivo three-dimensional neural circuit imaging system for small animal. In this system, two-photon excitation imaging techniques are combined with light-sheet microscopic imaging techniques. It provides complete information in aspects of cell biology, structural connectivity and functional circuit. The development of the imaging system is the main part of this project. It involves the research on imaging system, algorithms, software development, experimental evaluation and biomedical applications. Based on the requirements of in vivo deep and fast imaging for neural circuit, the imaging system takes the advantages of two-photon excitation imaging for deep imaging and light-sheet microscopic imaging for fast imaging. Furthermore, the imaging process and analysis platform are built to deal with fast imaging of neural circuit. The development of imaging system and performing pre-clinical biomedical applications can provide imaging methods and tools for studying the mechanism of neural circuit for small animal brian diseases model in vivo.