医药生物领域在分离纯化方面存在三大技术瓶颈：痕量活性物质的富集和纯化、手性化合物的拆分和蛋白样品的大规模纯化。高速逆流色谱是一种两相溶剂分配分离技术，与传统固体填料柱色谱相比较，具有100%回收率、洗脱方式灵活、可采用双水相体系而避免使用有机溶剂等优势。但是，现阶段逆流色谱仪器普遍较低的柱效限制了该技术的应用和发展。为突破“低柱效”这一关键技术障碍，本课题拟在长期理论和应用研究的基础上，通过考察多种仪器参数及色谱条件对柱效的影响，建立起适用于高速逆流色谱的柱效理论，并以此为指导进行新型高效高速逆流色谱的设计,制造出具有自主知识产权的超高分离效率的分析型和制备型新型高速逆流色谱仪器。本课题的实施将改变逆流色谱长期以来缺乏完整的色谱理论、柱效低的状况，为逆流色谱的改造及应用提供理论支撑并制备高分离效率的新型设备，充分发挥逆流色谱的技术优势，突破上述三个瓶颈问题，促进我国分离领域技术的创新发展。

At present, three major technical bottlenecks limit the development of separation and purification technology in the field of medical and biological areas, including the enrichment and purification of trace substances, the separation of mixture of chiral compounds and the large scale-up purification of protein samples. High speed counter-current chromatography is a two-phase solvent partitioning technique with 100% recovery rate, flexible elution mode and aqueous two-phase systems available to avoid the use of organic solvents compared with the traditional solid packing column chromatography. However, the low column efficiency of the counter-current chromatography instruments nowadays limits the application and development of this technology. To break through the key technical obstacle of "low efficiency", based on the long-term research in theory and counter-current chromatography application, this project will investigate the influence of various instrument parameters and chromatographic conditions on column efficiency, establish column efficiency theory suitable for high speed counter-current chromatography, and using the former research to guide the design and manufacture of new type high-performance high-speed counter-current chromatography. This project will fulfill the theory vacancy of the countercurrent chromatography, supporting the improvement and application of countercurrent chromatography, remarkably improves the separation efficiency of the instrument and therefore manufacture countercurrent chromatograph with super high column efficiency, taking full advantage of countercurrent chromatography technology, which can break through the three bottlenecks and promote the development of separation technology in China