以课题组精确测序并提交到NCBI数据库的一个火炬松叶绿体基因组序列为参考序列，采用高通量测序技术测定火炬松第一代核心育种群体混合样品的叶绿体基因组序列，经过比对分析，绘制火炬松叶绿体基因组个体水平突变图谱，筛选多态性位点，开发基于特异引物PCR的SNP标记，用于检测火炬松第二代核心群体的亲缘关系，验证多态性位点在完全谱系交配设计条件下世代传递的遗传稳定性，经验证的标记用于检测火炬松两个世代主群体的亲缘关系，建立育种群体亲缘关系图谱，确立亲缘关系在不完全谱系交配设计条件下在不同世代间的传递模式，作为群体亲缘关系调控的依据。本项目将全面阐明火炬松叶绿体基因组序列多态性以及在个体水平的变异模式，建立新型的松树育种群体亲缘关系调控技术体系，创新松树多世代轮回选择育种模式，对提高火炬松遗传改良水平具有重要意义。

Based on the previously accomplished sequence of loblolly pine (pinus taeda) chloroplast genome, which has been submitted to NCBI database and been released, in this project, mixed sample from the first-generation nucleus breeding population will be sequenced by using high-throughput sequencing technology, with the above mentioned submission as the reference sequence. After comparison, genetic diversity and variation in the chloroplast genome of loblolly pine will be profiled, and diversity loci will be screened. Featured primer PCR based single nucleotide polymorphism (SNP) markers will be developed with the high frequent mutation loci. The new developed SNP markers will be used to detect the second-generation nucleus population, in order to test the stability of the polymorphic loci among different generations under complete pedigree mating design. The tested SNP markers will be used to detect and profile the genetic relationship in the main populations of two generations and then, inheritance model of genetic relationship among different generations under incomplete pedigree mating design will be established. this will be the basis of regulation for the genetic relationship in the breeding population. The project will systematically explain the polymorphism and variation in the chloroplast genome sequence of loblolly pine, establish a new technology system to regulate the genetic relationship in the breeding populations of loblolly pine, innovate the model of multi-generation recurrent selection. This is significantly important to increase the level of genetic improvement in loblolly pine.