山杏是我国优良乡土树种,有望成为新型木本生物质资源。本项目以前期筛选的2份油脂及脂肪酸组分差异明显的山杏种质为试材，开展其不同发育期种子油脂含量及脂肪酸组成测定与代谢组学的比较分析，从中探究油脂累积及脂肪酸组分转化模式及其与代谢物变化间关系；构建均一DGE文库,开展Illumina-Solexa深度测序分析, 并运用iTRAQ蛋白质组学技术对供试山杏样品进行定量分析，进而确定山杏种子高品质油脂累积途径中关键调控基因及代谢路径；同时,开展高油脂密切相关代谢物的测定分析，并对不同发育期种子中调控脂肪酸累积相关的转录因子或代谢酶基因的转录表达进行检测与分析, 选择开展具有代表性关键调控基因的克隆及遗传转化拟南芥和表达检测分析研究，进而从转录组学、蛋白组学、代谢组学及代谢通路等不同层次阐明山杏种子高品质油脂累积的分子调控机制,这可为分子改良某些有经济价值的油料作物及生物质林木奠定重要基础。

Siberian apricot (Prunus sibirica L.) expected to a new type of woody biomass resources is an excellent native species. With the pre-screening, two Siberian Apricot Germplasms with the significant differences of the oil content and fatty acid composition as experimental materials are used to comparatively analysis for seed oil content, fatty acid composition and metabolomics in different developmental stages, and then the pattern of the accumulated oil and the transformed fatty acid composition and its relationship with the metabolite changes are explored. Uniformity digital gene expression (DGE) library is established and Illumina-Solexa deep sequencing is conducted subsequently, and isobaric tagging for relative and absolute quantitation (iTRAQ) is used to identify the dynamic profiles of proteins for developing seeds of Siberian apricot, which contribute to determine the Siberian apricot functional genes and metabolic pathways of the accumulated oil and the transformed fatty acid composition. Simultaneously, the metabolites and the transcripts of transcription factors and metabolic enzymes involved in oil accumulation and the conversion of fatty acid composition are analyzed in different developmental stages. Also the cloning of key oil accumulation-related genes, genetic transformation for Arabidopsis thaliana and expression analysis are performed. Our findings would help to elucidate molecular regulatory mechanism of increased oil production in developing Siberian apricot and to lay an important foundation for some economic oil crops and forest biomass improvement through molecular breeding in the future.