根际微生物释放挥发性有机物（mVOCs）介导微生物与作物间非直接接触互作是根际微生物与作物互作关系研究内容的重要组成部分，已成为当前根际微生物与作物互作关系研究的热点问题。本研究以“柘荣太子参2号”为材料，并利用从太子参连作土壤分离鉴定的病原尖孢镰刀菌和沙雷氏菌，研究特定病原微生物及根际微生物释放mVOCs介导连作太子参病害的化学生物学过程与机制。试验拟建立自然连作下收集分析根际mVOCs装置，动态检测不同连作条件下特定病原微生物和根际微生物释放mVOCs的组成成分，分析不同种植年限太子参根际mVOCs的时空成分变化规律及组成比例，明确对太子参生长发育产生影响的mVOCs具体组分，同时采用宏基因组测序技术解析这些组分对不同种植年限土壤根际微生物种群结构和数量的影响；进一步采用转录组测序技术分析太子参响应mVOCs各组分的潜在信号通路，并使用反转录PCR加以验证，阐明作用机理。

The interactions between plants and rhizospheric microbes mediated by microbial volatile organic compounds (mVOCs) are highlighted recently and become a hot issue for revealing the biological and ecological roles of mVOCs. To understand the chemical and biological process, and its underlying mechanism on continuous cropping Radix Pseudostellariae, the protocol is designed based on our previous studies. Zherong Radix Pseudostellariae 2 as well as specific Fusarium oxysporum and Serratia sp. isolated from its rhizosphere soil were used as experimental materials to study the biological and ecological roles of their secreted mVOCs in continuous cropping Radix Pseudostellariae. The test system for collecting and anlyzing rhizospheric mVOCs would be designed based on natural continuous cropping conditions. The dynamics of mVOCs from specific pathogenic or rhizospheric microorganisms under different continuous cropping conditions would be determined along with the analysis of their spatial and temporal changes. Furthermore, the effect of rhizospheric mVOCs on the growth and development of Radix Pseudostellariae would be investigated along with their effect on the number and strucuture of rhizospheric microorgansim communities, in order to confirming the positive components of mVOCs secreted by rhizospheric microorgansims. Based on the research mentioned above, transcriptome analysis of Radix Pseudostellariae would be carried out for identifying underlying signal pathway responsed to the positive mVOC component, and RT-PCR would be employed to comfirm the related genes of signal pathway. It is expected to furhter uncover the biological role and its underlying mechansim of rhizospheric mVOC for continuous cropping obstacle.