角膜内皮炎是一种特发性的角膜内皮急性非化脓性炎症。由于近些年来其发病率明显增加，疾病发展迅速，且预后不佳、严重影响患者视力，而逐渐被人们所重视。临床研究表明，角膜内皮炎的发病与病毒感染及免疫反应有关，其中以巨细胞病毒性角膜内皮炎的病情最为严重，在亚洲人群中的发病率也显著高于其他人种。该病的临床表现多样，确诊依赖于房水聚合酶链式反应等实验室手段。然而，临床认识不足，再加上诊断方法尚未普及，导致误诊误治的情况极为常见，致盲率较高。. 本课题试图以小鼠建立巨细胞病毒性角膜内皮炎的动物模型，通过随机对照实验的方法，动态观察小鼠在角膜内皮炎发病过程中不同时间点的眼部及组织病理表现；利用聚合酶链式反应、酶联免疫吸附测定等技术，监测小鼠眼部的病毒抗原、抗体的水平。探讨角膜内皮炎的发病机制，为临床诊断、治疗提供思路。

Corneal endotheliitis is an idiopathic disease, mainly presented as acute, non-suppurative inflammation of corneal endothelia. It has drawn more attention in recent years due to its increasing prevalence, rapid progression and poor prognosis. Researches show that viral infections and subsequent immune responses are involved in the pathogenesis of endotheliitis. Cytomegalovirus, which is highly prevalent in Asian populations, accounts for one of the most severe forms of corneal endotheliitis. The detection of virus by aqueous humor polymerase chain reaction is mandatory in the diagnosis of endotheliitis, because clinical manifestations are diversified. However, without sufficient recognition of the disease or application of viral tests in clinical practices, patients are often misdiagnosed and likely to suffer from blindness.. In this study, we attempt to develop a murine model of cytomegalovirus corneal endotheliitis. Using randomized controlled methods, we monitor the clinical and pathological features at different time point after infection, as well as the virus load and neutralizing antibodies by polymerase chain reaction and enzyme-linked immunosorbent assay. With these experimental data, we will be able to further investigate the pathogenesis of corneal endotheliitis, and find new solutions for the disease in clinical diagnosis and treatment.

角膜内皮炎是一种特发性的角膜内皮急性非化脓性炎症。由于近些年来其发病率明显增加，疾病发展迅速，且预后不佳、严重影响患者视力，而逐渐被人们所重视。临床研究表明，角膜内皮炎的发病与病毒感染及免疫反应有关，其中以巨细胞病毒性角膜内皮炎的病情最为严重，在亚洲人群中的发病率也显著高于其他人种。该病的临床表现多样，确诊依赖于房水聚合酶链式反应等实验室手段。然而，临床认识不足，再加上诊断方法尚未普及，导致误诊误治的情况极为常见，致盲率较高。本课题试图以小鼠建立巨细胞病毒性角膜内皮炎的动物模型，通过随机对照实验的方法，动态观察小鼠在角膜内皮炎发病过程中不同时间点的眼部及组织病理表现；利用聚合酶链式反应、酶联免疫吸附测定等技术，监测小鼠眼部的病毒抗原、抗体的水平。探讨角膜内皮炎的发病机制，为临床诊断、治疗提供思路。