锰（Mn）采选冶联合矿山企业导致周边农田土壤中以Mn为主的重金属含量过高，造成农产品Mn超标，有效阻控农作物吸收Mn成为一项亟待解决的农业难题。目前，针对农作物重金属吸收关键期进行精准阻控的系统研究尚少。项目通过盆栽试验研究玉米、大豆在不同水平Mn污染土壤中的生长动态及Mn累积规律，确定玉米、大豆吸收Mn的关键期；筛选优势微生物，并改性生物炭，研究适时适量施用微生物-改性生物炭对玉米、大豆累积Mn的联合阻控效应；利用高分辨率微电极测量系统原位分析微生物-改性生物炭对Mn胁迫下玉米、大豆根际微环境的改变及其对Mn迁移转化的影响，探究微生物-改性生物炭对玉米、大豆吸收Mn的根际阻控机制和玉米、大豆的逆境生理生化响应机理；通过大田试验验证施用微生物-改性生物炭对农作物吸收复合污染土壤中Mn的精准阻控效应。项目以期获得一种精准阻控农作物吸收Mn的科学方法，为农作物的安全种植提供有效途径。

Excessive Mn in crop is caused by Mn polluted farmland around the united enterprises of mining-dressing-melting. How to block Mn uptake of crops has become an urgent problem. The systematic study on precise blocking Mn uptake of crops at the key growth stage is very scarce. In order to ascertain the growth dynamics and the key stage of Mn uptake by crops, corn and soybean are studied by pot experiments under different Mn levels. Based on the key stage of Mn uptake by corn and soybean, the optimal quantities of selected suitable microbes and modified biochar will be used to block Mn uptake in the appropriate periods. The effects of microbes-modified biochar on Mn accumulation in corn and soybean will be analyzed. Meanwhile, a motorized microprofiling system equipped with microsensors is used to detect changes of rhizosphere microenvironment in situ and the influence on Mn migration. The results will reveal the mechanism of Mn uptake blocking of corn and soybean by rhizosphere microenvironment change and physiology responses of corn and soybean. Finally, the effects of microbe-modified biochar blocking Mn uptake by corn and soybean will be clarified in field experiments. In this study, the scientific method that could accurately block Mn uptake by corn and soybean will be obtained, which will provide a new solution for safe planting crops in Mn polluted farmland.