作为我国花生的主要灾害之一，渍涝在南方花生生育前期频繁发生。通过持续15年的不断探索，我们在确定渍涝的生理与农艺影响、建立渍涝鉴定指标与标准、耐渍生态育种、创建近900份种质的世界首个耐渍鉴定数据库、灾害栽培防控等方面取得了系列进展。然而，国内外花生渍涝研究多囿于一般性的表型分析，缺乏原创性的深层次探讨。本项目拟聚焦渍涝危害最为直接、持久、严重的根系部位，采用植物形态与解剖学方法，研究渍涝胁迫下耐性差异明显的两类花生品种的主根、侧根、不定根的形态特征、组织结构、细胞与亚细胞特点；采用iTRAQ定量蛋白质组学技术、LC-MS/MS液质联用质谱技术、生物信息学技术和real time RT-PCR技术相结合的方法，研究三类根的差异蛋白质及代谢途径、耐涝相关基因的表达与验证，进而剖析形态解剖分异与蛋白质组、基因表达变化的对应关系，基本探明耐渍花生的主要机理，为花生渍涝灾害治理提供理论依据。

Waterlogging, as one of main disasters in peanut production in China, occurred frequently in the earlier growing stage in southern areas. To solove this problem, we have developed many achievements in past fifteen years, such as determining physilogical and agronomic effects, establishing indexes and criteria for disaster identification, creating the first database of tolerance identification including near 900 germplasm, waterlogging precautioning and controling with cultivation performance. Although the previous researches around the world carried out common phenotypic analysis for peanut waterlogging, these studies were insufficient in originality and thoroughly discuss. This projepct will focus on roots where waterlogging stress directly, persistently and seriously effects on peanut plants. Morphological characteristics, tissue structures, cellular and subcellular characters of main root, lateral roots and adventitous roots of typical tolerant and susceptible accessions will be studied by morphological and anatomical methods. Further, indentification of differential proteins, pathway analysis for these proteins, expression and verification of tolerant-related genes will be also explored by quantitative proteomics (iTRAQ) technique, LC-MS/MS technique, bioinformatics technique and real time RT-PCR. Hence the relationships between morphological characteristics and proteome of roots will be analysed, which wll illuminate tolerance mechanisms and provid theoretical basis for waterlogging control of peanut production.