经典文献、临床和实验研究均表明天枢穴具有双向调节肠运动（腹泻和便秘）的效应，但研究中未区分左、右天枢的效应差异。胃肠神经生物学的提示，左、右天枢穴区下的神经支配存在明显差异；临床深刺天枢突破腹壁具有疗效优势，而这种深刺在左、右穴涉及的组织具有显著的不同。本项目提出“针刺左右天枢穴由于传入途径的差异形成不同的刺激-响应模式，可以对肠的不同部位产生不同方向的效应、针对穴区下不同组织（深度）的针刺可能产生特定部位的优势效应”的观点。以正常大小鼠、交感神经受体β1β2基因敲除小鼠、副交感神经受体M2M3基因敲除小鼠为研究对象，通过观察手针/电针深刺天枢、穴区刷毛、刺激皮肤、肌肉、腹壁、肠壁等干预方式，观察远、近端结肠、回肠的运动变化，观察内脏大神经/盆神经节/迷走神经活动的变化，以验证项目的观点，建立天枢穴刺激-响应的模式，以深化天枢穴双向调节的科学内涵提高临床疗效。

It is indicated that ST25 constitudes bi-directional regulational effect for treat diarrhea and constipation based on classic works, clinic and basic research, but the differenct between the left and right ST25 is not reveal. According to neurobiology of gastrointestine, the innervation between the left and right ST25 is different. Punctureing at ST25 deeply through abdominal wall shows advanced effect in clinic, and the difference tissue of different side of ST26 should be considerd. Our study raise the hypothesis that different stimuli-responce pattern, sourced from afferent of the left and right ST25, could induce different effect toward different segmental intestine, and puncturing at different tissue beneath the acupoint may produce specific advance. Using normal rats/mice, gene-knockout mice(β1β2-/- and M2M3-/-) and different intervention (brush, stimuli at skin, mucle, belly wall, or bowel wall), we will observe the motility of distal, proximal colon and ileum, and the spike of major splanchnic nerve, pelvic ganglion and vagus to valify the hyposis, so as to establish the stimuli-responce pattern of ST25, deepen the scientific connotation of Tianshu of bidirectional regulation, and improve the clinical efficacy.