隐尾蠊是一类特殊的食木性蜚蠊,生活于冷凉、潮湿的环境。该类蜚蠊是联系白蚁和其他蜚蠊类群的纽带,无翅却间断分布在亚洲和北美,成为近年来网翅总目系统发育和生物进化研究的热点。隐尾蠊间断分布在我国的秦岭－大巴山区、川西－横断山区、东北山区，物种调查甚少,各区隐尾蠊的物种多样性及亲缘关系亟待调查和澄清；隐尾蠊的起源、演化和传播路径有待进一步阐释。本项目拟对我国隐尾蠊进行调查,弄清隐尾蠊物种多样性水平和分布格局,综合比较染色体数目特征及隐尾蠊雌性生殖器特征的分类应用价值;综合运用自测和基因库下载的隐尾蠊分子数据,确定COI基因片段作为隐尾蠊DNA 标注条形码的可行性;使用线粒体基因组结合18S、28S基因对世界隐尾蠊进行系统发育分析,探讨其亲缘关系, 采用化石标定及进化速率的分子钟研究方法估算分化时间,利用重建祖先分布地的方法推测其进化路线，解释隐尾蠊现今分布格局。

The woodroach Cryptocercus is one kind of xylophagous insects which live in mountain forests of temperate and moist regions. The taxonomic status of Cryptocercus is very special and important owing to its link connecting termites and other cockroach species. The apterous Cryptocercus exhibits a disjunct distribution in Asian and North America, which is the main reason for the hotspot research in molecular phylogeny and biological evolution of Dictyoptera. In China, Cryptocercus cockroaches show a disjunct distribution in Qinling-Daba mountains, western Sichuan-Hengduan mountains and mountain area of Northeastern China. Cryptocercus in these area was rarely investigated, and the biodiversity of species and phylogenetic relationship kept unknown. Its origins，evolution and immigration need to be further clarified. The goals of this project are to: 1) investigate the biodiversity of Cryptocercus, and figure out the diversity and distribution pattern of Cryptocercus in China; 2) synthetically compare and value the application of the chromosome number and female genitalia in classification; 3) assess whether COI gene fragment could serve as a standard barcoding to delimit species; 4) perform the phylogenetic analysis based on mitochondrial genome combined with nuclear genes 18S and 28S , and discuss the phylogenetic relationship of Cryptocercus worldwide; 5) calculate the divergence time by means of fossil calculation and molecular substitution rate, and infer the biogeographical reconstructions and explain the cause of current distribution pattern in order to establish the foundation for further study in phylogeny and evolution of Blattodea.