野螟亚科是鳞翅目草螟科的一类常见昆虫，尽管基于形态和分子信息的研究证实其为单系群，但是亚科内属间的系统发育关系尚未报道，争议属的分类处理还存在许多问题。申请人前期依据形态特征并使用分支分类学方法建立的野螟亚科系统发育树未能解决所有属的系统发育关系，一些争议属的分类位置仍无法确定。本申请项目拟选取野螟亚科各属、种作为内群，以斑野螟亚科等类群作为外群，以最大简约法依据形态特征建树，与以最大似然法和贝叶斯推断依据2个线粒体基因（COI、16s RNA）和6个核基因（EF-1α、wingless、CAD、MDH、GAPDH、RPS5）建树进行联合分析。拟探究野螟亚科各类群之间的系统发育关系；确定争议属、种的分类位置；并建立常见种和重要种的DNA条形码数据库。为深入理解螟蛾总科及鳞翅目昆虫的谱系进化关系提供基础数据，为近缘种、疑难种和隐存种的鉴定提供依据，为农林业和植物检疫害虫的鉴定提供便利。

Pyraustinae is a common subfamily in Crambidae of Lepidoptera. The monophyly of Pyraustinae is well supported by phylogeny analyses based on both morphology characters and molecular data. And based on morphology characters and methods of cladistics, the phylogeny of Pyraustinae was analysed by applicants. But there are still some questions about phylogenetic relationships of genera and systematic position of disputed genera need to be answered. In this study, with species and genera of Pyraustinae as ingroup taxa, Spilomelinae and other subfamilies as outgroup taxa, based on the morphology characters and sequences of mitochondrial gene COI and 16s RNA, nuclear gene EF-1α, wingless, CAD, MDH, GAPDH, RPS5, phylogenetic trees of Pyraustinae are constructed with the maximum-parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI) methods. The focus of this study is to reconstruct a phylogenetic hypothesis of Pyraustinae and stable systematic position of disputed genera. The resulting phylogeny is used to investigate the evolution of Pyraloidea and Lepidoptera. At the same time, DNA barcoding data of common and economic species is established and used to identify species in the work of plant protection and plant quarantine.