菊花是我国十大传统名花和世界四大切花之一。多数菊花品种属典型的短日照植物。目前主要通过光温调控以实现周年生产供应，能耗成本高昂。我们前期研究发现超表达CmMYB2转基因拟南芥花期改变，超表达CmMYB2转基因菊花开花提前，但其调控花期的具体机制尚不清楚；通过酵母双杂交发现CmMYB2与CmMYB9A互作。本研究拟通过CmMYB2转基因株系表达谱分析鉴定其调控的下游基因；通过酵母双杂交进一步从菊花文库中筛选CmMYB9A的互作蛋白，从拟南芥文库中筛选CmMYB2互作蛋白，并运用双分子荧光互补技术（BiFC）、GST Pull-down技术和免疫共沉淀技术（CoIP）进行验证，再通过转基因手段对筛选出的目的蛋白基因进行功能鉴定。最终阐释菊花CmMYB2蛋白复合体调控花期的分子机制。该研究不仅能丰富植物MYB转录因子调控花期的理论基础，也可为菊花花期改良提供基因资源，具有重要科学意义和应用前景。

Chrysanthemum is an important ornamental plant across the world. It is a typical short-day (SD) plant that responds to shortening day length during the transition from the vegetative to the reproductive phase, which challenges the sustainable chrysanthemum producing due to high energy cost. CmMYB2 changed flowering of its overexpressed Arabidopsis, while induced flowering of its overexpressed chrydanthemum, but the mechanism involved in regualting flowering time remained unclear. In present proposal, we aim to uncover the molecular mechanism of CmMYB2 in regulating the flowering time of chrysanthemum and Arabidopsis by identifing the downstream genes via gene expression analysis, using yeast two-hybrid to screen CmMYB9A’s interaction proteins in chrysanthemum based on its interaction with CmMYB2 in vivo, CmMYB2’s interaction proteins in Arabidopsis, then using bimolecular fluorescence complementation (BiFC), and GST Pull-down and Co-Immunoprecipitation（CoIP）to confirm the interaction in vitro and in vivo. We will investigate the function of screened interaction protein using over-expression transgenic system in both Arabidopsis and chrysanthemum. Taken together, the present proposal will disclose the mechanism of CmMYB2 complex in regulating the flowering time of chrysanthemum, enrich the understanding of how MYB play roles in flowering time regulation, which will provide the candidate genes for improvement of chrysanthemum flowering time. Therefore, the present proposal is of both theoretical and practical significance.