申请人针对肿瘤免疫治疗重大需求，致力于发展活体跨层次、多分子并行检测和靶向标记的光学成像新方法。发明理化性能优异的红色荧光蛋白功能探针，克服并行检测时光谱串扰严重的问题，建立了活细胞内多分子同步成像新方法。发明八价多肽纳米探针确证了新型肿瘤靶向治疗多肽，建立有望用于人体的超小粒径仿生纳米平台，解决了治疗性多肽在活体内安全运输和实体瘤内有效扩散的难题。现主持NSFC重大研究计划重点支持项目“活体跨层次整合成像研究肿瘤肝转移的区域免疫”。近五年，在Autophagy和ACS Nano等期刊上发表第一或通讯作者SCI论文16篇（3篇IF>10）。发明的探针和方法被国际同行应用于生物分子成像，已与剑桥大学和哈佛大学等22家单位签署转让协议，使用者的论文在PNAS(3篇)上发表。应邀成为SPIE Photonics West(美国)“生物光子学与免疫应答”会议的委员会成员，担任分会主席并做邀请报告。

Aiming at the significant demand of tumor immunotherapy, the applicant devotes myself to the development of innovative optical imaging methods characterized with multi-level, multi-molecular parallel detection, and targeting labelling in vivo. The developed red fluorescent proteins and functional probes, with excellent physical and chemical properties, solved the problem of the serious spectral crosstalk in multi-molecular parallel detection and were used to establish a novel method of synchronous optical imaging for multi-molecular events in single living cells. The applicant developed an octavalent-peptide nanoprobe to validate a novel tumor targeting therapeutic peptide, and established an ultra-small peptide-lipid biomimetic nanoplatform, which overcame the bottleneck problem of therapeutic peptides in vivo transportation safety and effective diffusion in solid tumors. The applicant is in charge of a Key Support project in NSFC Major Research plan, “In vivo cross-level integration imaging of regional immunity of tumor liver metastasis”. In recent five years, the applicant has published 18 papers, either as first author or corresponding author, in SCI-indexed/international journals, such as Autophagy and ACS Nano. Moreover, twenty-two research affiliations (e.g., University of Cambridge and Harvard University) has signed the material transfer agreements (MTA) with the applicant. They utilized those probes widely in biomolecular imaging, which 3 papers have been published on PNAS. Additionally, the applicant has been invited as a Program Committee member and Session Chair for SPIE Photonics West conference (USA) “Biophotonics and Immune Responses” and has given invited oral reports.