感应环境胁迫调节开花时间对植物生长发育极为重要，其分子机制在模式植物拟南芥上已有研究，但在多年生的常绿果树上仍未研究清楚。荔枝是华南地区广泛栽培的常绿果树，其成花诱导需要冬季的低温胁迫。本课题组过去研究表明，低温诱导成花过程中也诱发胁迫信号分子活性氧（ROS）的积累，外源ROS诱导剂处理可以减少荔枝成花对低温的需求。本项目从低温和ROS的调控为切入点，利用高通量测序技术进行转录组分析，并利用资源圃中低温需求不同的荔枝资源进行成花过程的表达分析，以筛选荔枝中响应ROS、低温且参与成花诱导的基因。本项目还通过候选基因的生物学功能分析、ChIP-seq技术筛选转录因子调控的靶基因，以及利用酵母双杂交技术进行蛋白互作分析，解析ROS参与的胁迫诱导荔枝成花的基因调控网络，为探索胁迫调控常绿果树开花的分子机理积累基础资料，也为应对全球气候变暖、培育适应气候变化的荔枝新品种提供基因储备。

Stress-induced flowering is essential for plant growth and development. Its molecular mechanism in Arabidopsis has been studied. However, its mechanism in the perennial ever green fruit trees is unclear. Litchi is widely cultivated in Southern China. Flowering in litchi is induced by low temperature in winter. Our previous studies showed that reactive oxygen species (ROS) accumulate under low temperature stress and during the floral induction stage. ROS inducers can enhance flowering under less stressful conditions. In this project, we focus on these signaling events. Litchi transcriptome analysis will be carried out, and gene expression of litchi with different low temperature requirement will be analyzed to identify genes involved in temperature sensing, ROS response and floral induction. We will also examine the biological function of the candidate genes, map target of transcription factors by ChIP-seq, and analyze interaction proteins by yeast two hybridization. This project aims to clarify flowering regulatory network of ROS and stress in litchi, to provide vital information to clarify a general molecular mechanism in evergreen fruit trees, and to provide important candidate genes for breeding cultivars adapted to global warming.