涝害的实质是由低氧胁迫造成的，耐涝植物可通过改变呼吸代谢途径、形成通气组织等方式应对低氧逆境，降低危害程度。樱桃抗涝性较弱，短时间淹水即会造成严重的生理伤害，甚至死树。针对樱桃易受水涝危害、伤害机理不清的问题，以生产中应用较多、起源不同的4种樱桃为试材，进行低氧胁迫试验。以根系为中心，从线粒体结构、呼吸代谢生理水平、基因表达及根构型等方面，研究低氧造成根系伤害的发生次序及程度，筛选出对低氧敏感的生理指标。通过无参考转录组及数字基因表达谱（DGE）测序分析，查找参与调控樱桃根系呼吸代谢、与低氧胁迫密切相关的差异表达基因，分析其执行的主要生物学功能。结合低氧敏感生理指标的变化，筛选PDC等关键酶基因，利用qRT-PCR技术进行关键基因的相对表达量分析，验证其在低氧胁迫下基因网络中的功能及表达模式。最终，揭示樱桃根系呼吸代谢响应低氧胁迫的生理与分子机制，阐明低氧造成根系生理伤害的主要环节及原因。

Waterlogging damages the crop by causing hypoxia stress. In order to reduce the harm degree, plants with high waterlogging tolerance protected themself from hypoxia stress by changing respiratory pathways, forming aerenchyma and so on. Cherry is the fruit tree with low waterlogging tolerance. Serious physical injuries, even death would caused by even a short time of flooding.Although cherrys are easily to harmed by waterlogging, the damage mechanism is still not clear. Concerning over this problem, four widely used rootstocks which originated different are selected as materials to carry out experiments under hypoxia stress. To ensure the sensitive indicator to hypoxia stress in cherry, the order and degree of root harmed by hypoxia are demonstrate from the changing of subcellular structures such as mitochondria, respiratory metabolism physiological levels and morphology such as root architecture.Finding differentially expressed genes involved in the regulation of cherry root respiration metabolism and closely related to hypoxic damage though non parametric transcriptome sequencing method and digital gene expression profiling (DGE) analysis. Then analysising main biological function of the gene. Combining with the changing of sensitive physiological indexes, screening gene of key enzymes such as PDC. Analysising the relative expression of key genes by using the qPT-PCR technology, then verifying that the function and expression patternof the gene in the network of the gene under hypoxia stress. Finally, revealling the physiological and molecular mechanisms of cherry root respiration metabolism responsing to hypoxia stress, then elucidating the main process and reason that cherry root physiological damage caused by hypoxia.