不同质地桃品种在果实耐贮性方面存在明显差异，而其机制尚未完全阐明。本项目拟以乙烯信号上游的SEP型MADS基因PpSEP1转录因子为切入点，采用质地不同的三个桃品种为试材，在分析成熟软化过程中不同质地桃果实PpSEP1基因表达、蛋白积累量，以及耐贮性相关基因表达量变化的基础上，利用VIGS技术诱导溶质桃果实中的PpSEP1基因沉默和在番茄突变体rin中异位过表达PpSEP1基因，明确PpSEP1在不同质地桃果实耐贮性形成中的功能；同时研究不同质地桃品种中的PpSEP1基因转录活性的差异，并进一步通过凝胶迁移率和酵母单杂交技术分析PpSEP1与耐贮性形成关键基因启动子序列的作用元件结合情况，阐明PpSEP1对耐贮性形成关键基因的转录调控机制。通过以上研究以期阐明PpSEP1对不同质地桃果实耐贮性状形成的调控机制，从而为全面阐明桃果实的成熟软化机制以及高效培育耐贮品种提供理论依据。

The peach fruits with different textures showed significant differences in fruit storability, however, its mechanism remains to be fully elucidated. In this project, as the transcription factor of ethylene upstream signaling, PpSEP1, a member of SEP type MADS gene familigy, will be surveyed, using three peach cultivars with different texture as materials. PpSEP1 gene expression, protein accumulation, as well as the expression level of genes related to storability in different textures peach fruit during ripening and softening will be analyzed. Furthermore, the biological functions of PpSEP1 in storability formation of peach fruit will be studied through gene silencing by VIGS technique in melting peach fruit and ectopic overexpression in tomato rin mutant . In addition, PpSEP1 gene transcriptional activity will be compared in peach varieties with different textures, and the binding between PpSEP1 and promoter of storability key genes will be investigated by gel mobility and yeast one-hybrid technology. Through these studies, the regulatory mechanism of PpSEP1 in the forming of storability trait in peach peach fruit with different textures will be further elucidated, which can provide a theoretical basis for the comprehensive understanding of ripening and softening mechanism in peach fruits and breeding of storable peach varieties with high efficiency.