SRBSDV是白背飞虱传播的一新病毒，水稻早期感染该病毒后严重减产。课题组前期已确定该虫对病、健稻苗挥发物的趋性行为不同，即毒虫优先选择无毒苗而无毒虫倾向于选择毒苗。是因该病毒侵染改变了水稻挥发物成分，还是该病毒的侵染改变了该飞虱的嗅觉系统认知网络导致这奇特现象的发生？还不可知。本项目拟研究病、健稻苗挥发物中活性成分，探明SRBSDV诱导稻苗挥发物中信息化合物的变化；结合转录组学方法，克隆鉴定该虫OBPs基因，研究OBPs与活性成分的结合特性，阐明关键性OBPs在该虫寄主植物定位中的功能，回答上述科学问题。该研究结果将促进从行为学角度和分子水平阐明SRBSDV调控该虫趋性行为来加速该病毒传播的行为调控机制，为防控该虫和SRBSDV提供重要的理论基础和技术支持，从协同进化的角度理解有利于该病毒传播的SRBSDV-白背飞虱-水稻的互作关系。

SRBSDV(Southern rice black-streaked dwarf virus) is a novel virus, Sogatella furcifera is the transmission vector of SRBSDV. In our previous study, chemotaxis and host plant orientation behavior of S. furcifera between SRBSDV-infected and healthy rice seedlings were significant different. The purpose of this study is to isolate and identify the semiochemicals of plants volatite from the SRBSDV-infected and healthy rice seedlings, to investigate SRBSDV whether induce the change of semiochemicals from rice seedlings or not; It is ascertain that SRBSDV whether injury/inhibit the antenna and olfactory celles by using GC-EAD and GC-SCR or not. OBPs genes of this insect are cloned, correlations beweent OBPs genes and host orientation behavior of this insect are elucidated; In order to elucidate the funcation of OBPs on host plant orientation of S. furcifera, prokaryotic expression of OBPs genes are performed to give OBPs, the binding characteristics of OBPs and the infochemicals are analysed. From the perspective of insect ethology, this project is to elucidate how SRBSDV manipulate the orientation behavior of S. furcifer to enhance its transmission efficiency, to illustrate the manipulation mechanism on molecular level, to explore the manipulation technique of chemical communication by inhibiting the function of OBPs, and to provide theoretical and technical bases for controlling SRBSD and its vector. Moreover, it is important and helpful to understand the interaction of SRBSDV-Vector-Rice seedlings in coevolution.