糖尿病性溃疡已成为糖尿病患者致残和死亡的主要原因。血管内皮细胞损伤、新生血管形成障碍，氧化应激损伤等是糖尿病足难愈性溃疡局部微循环病变的因素。课题组应用回阳生肌膏治疗糖尿病性溃疡20余年，取得了良好的临床疗效。本课题以糖尿病溃疡患者原代微血管内皮细胞及糖尿病溃疡大鼠模型为研究对象，阐明回阳生肌膏治疗前后对糖尿病溃疡患者及大鼠靶皮损血管新生，CD105、VEGF/VEGFR等血管生成相关细胞因子和炎症因子，以及血清血管生成相关细胞因子的干预机制；探讨回阳生肌膏主要成分对体外糖尿病溃疡创面原代微血管内皮细胞增殖、迁移、微管腔形成及Pi3k/akt血管生成信号转导通路的影响，揭示其促进血管新生的作用机制。验证并丰富“煨脓以长肉”、“回阳以生肌”理论：“煨脓”以抑制炎症反应，改善乏氧状态、缓解氧化应激损伤；“回阳”以促进血管新生、改善微血管状态，加快溃疡愈合。

Diabetic ulcer has become the major cause of death and disability in patients with diabetes mellitus. Injury of vascular endothelial cells and neovascularization disturbance and oxidative stress injury is factors of diabetic foot refractory ulcerative microcirculation lesion. The research group used Huiyang Shengji ointment in the treatment of diabetic ulcers for 20 years, and achieved a good clinical efficacy. This topic to diabetic ulcers in patients with primary microvascular endothelial cells and diabetic ulcer rat model as the research object, clarify Huiyang born muscle cream before and after the treatment of ulcer of diabetic patients and diabetic ulcer rat target lesions angiogenesis, CD105, VEGF / VEGFR and other angiogenesis related cell factors and inflammatory factors, and the level of serum vascular generation related cytokine intervention mechanism; of Huiyang Shengji ointment main component of in vitro diabetic ulcer wound primary micro vascular endothelial cell proliferation, migration and microtubule cavity formation and blood vessels in the Pi3k/akt generation signaling pathway, reveal its action mechanism to promote angiogenesis. To verify and enrich "suppuration to flesh" and "back to yang to muscle" theory: "simmer septic" to suppress the inflammatory reaction, improve hypoxia, alleviate the oxidative stress injury; "Huiyang" to promote angiogenesis and ameliorate the tiny blood vessels, accelerate ulcer healing.