基于“化瘀软坚解毒法”治疗瘢痕疙瘩的临床经验，课题组进一步研究证明化瘀软坚解毒法能抑制瘢痕疙瘩成纤维细胞异常增殖，但其具体机制尚不清楚。近期研究发现miR-21及mTOR信号通路在瘢痕疙瘩形成过程中具有重要作用，而且miR-21的靶基因PTEN是mTOR信号通路负反馈调节因子。据此，本项目从探讨miR-21对mTOR信号通路的调控角度来研究化瘀软坚解毒法抑制瘢痕疙瘩增生的细胞生物学机制。项目以人瘢痕疙瘩和正常皮肤组织、瘢痕疙瘩裸鼠模型、人瘢痕疙瘩成纤维细胞为研究对象，化瘀软坚解毒法为干预手段，从组织-模型-细胞三个层次来研究mTOR信号通路关键分子在瘢痕疙瘩组织及其成纤维细胞中的表达情况及miR-21的调控作用和机制，同时探讨化瘀软坚解毒法的干预作用和靶点。预期本项目的研究，将会进一步揭示瘢痕疙瘩成纤维细胞异常增殖的分子机制，明确化瘀软坚解毒法抑制瘢痕疙瘩增生的相关机理。

Based on clinical experience of Huayu Ruanjian detoxification method treating keloid, further studies have shown that Huayu Ruanjian detoxification method can suppress the abnormal proliferation of keloid fibroblasts, but the exact mechanism is unclear. Recent studies have found that miR-21 and mTOR signaling pathway plays an important role in the process of keloid formation, and the miR-21 target gene PTEN is negative feedback regulator of mTOR signaling pathway. Accordingly, by investigate miR-21 regulating mTOR signal pathway,this project research the cell biological mechanisms of Ruanjian detoxification method to inhibit the proliferation of keloid. The project, using human keloid and normal skin tissue, keloid nude model, human keloid fibroblasts as for research subjects, and Huayu Ruanjian detoxification method to intervene, from the tissue-model-cell three levels to study mTOR signaling pathway molecules expression in keloid tissue and fibroblasts, and the regulation of miR-21 and its mechanism, as well as explore the intervention and targets of Huayu Ruanjian detoxification method. The study is expected to further reveal the molecular mechanism of keloid fibroblasts abnormal proliferation, and determine the specific mechanism of Huayu Ruanjian detoxification method inhibiting keloid proliferation.