前期研究表明，血燥证是银屑病常见的难治证候之一，临床常表现为斑块型，课题组前期研究表明养血解毒方治疗斑块型银屑病血燥证安全有效，可以通过抑制VEGF而抑制银屑病微血管的增生。本课题拟以survivin如何调控VEGF，诱导微血管消退为切入点，以临床中疗效确切病例的靶皮损组织为主要研究对象，观察养血解毒方治疗前后银屑病患者靶皮损表达survivin以及Survivin/PI3K/βcatenin蛋白的变化；并通过survivin过表达小鼠和K14/VEGF 银屑病样转基因小鼠观察该方对其皮损血管形态及survivin、VEGF下游通路的影响；另以中药含药血清对survivin过表达的内皮细胞增殖、凋亡、细胞周期变化、迁移、管腔形成及通路蛋白的影响，以探明养血解毒方对survivin、VEGF及相关信号通路的影响，揭示其抑制血管新生及促进微血管消退的作用机制，丰富银屑病从“血”论治理论。

Previous studies have shown that blood dryness is one of the basic syndromes of psoriasis, which mainly are plaque psoriasis. Yangxuejiedu decoction was effective to blood dryness syndromes in plaque psoriasis, and VEGF was inhabited. This research is designed to find why and how VEGF and angiogenesis depressed. The psoriasis specimens obtained before and after the treatment through a randomized, double-blind, placebo-controlled trials, which would be treated by Yangxuejiedu decoction, and survivin, PI3K/Akt-β-catenin-Tcf/Lef will be detected. Survivin overexpression and K14/VEGF psoriasis-like transgenic mice raised by Yangxuejiedu decoction, will be used to observe the expression of survivin, PI3K/Akt-β-catenin-Tcf/Lef in different stage. PMA-induced endothelial cells in vitro angiogenesis and to observe the proliferation, migration, micro-lumen formation and signal transduction pathway of survivin, PI3K/Akt-β-catenin-Tcf/Lef, VEGF expression in containing serum and to find how angiogenesis and apoptosis are regulated.