有些寄主昆虫被外来生物寄生后行为会发生变化，这一现象广泛存在于许多寄生生物—寄主的相互关系中，被核型多角体病毒（HaSNPV）感染的棉铃虫上爬至植株上部倒挂死亡就是外来生物改变寄主行为的典型例子。本项目在温室内已经证实被病毒感染的棉铃虫具有上爬行为的基础上，继续系统研究感染病毒棉铃虫的行为变化，主要通过吊绳在室内研究这种行为，构建一种便于操作、容易观察的技术模型；再分别通过注射多巴胺兴奋剂或抑制剂上调或下调感病棉铃虫重要神经递质多巴胺滴度监测棉铃虫的行为变化；最后通过转录组测序筛选多巴胺受体、转运体、多巴胺脱羧酶等关键酶基因，用RNAi技术研究这些多巴胺代谢途径上的关键基因在行为变化中的作用，通过这些研究可以明确多巴胺在感病棉铃虫行为变化中的作用机理，从神经递质角度对这种古老的现象给予一定解释，为进一步理解这种现象提供一些基础知识。

There are some interesting phenomenons that the normal behaviors of some insect infected by biological factors are changed in the interaction of parasitoids and host. The cotton bollworm larvae infected by HaSNPV usually climb up and die on the top of plants. The phenomenon was improved in our preliminary research in the greenhouse. In the proposal, at first we will study the behavior of cotton bollworm larvae infected by HaSNPV and build a model to study the behavior in the further experiments. We increase or decrease the amount of dopamine by injecting some stimulant or inhibitor of dopamine, and then the behavior of treated cotton bollworm will be studied using the model. We will conduct the transcriptome sequencing of infected cotton bollworm by HaNPV. Some dopamine receptor genes, the dopamine transporter genes, dopamine decarboxylase will be choosed and their function in the behavior change will be studied by RNAi. The role of dopamine in the behavior change of cotton bollworm infected by HaNPV will be clarified by the proposal. We hope the results will help to understand the interesting phenomenon based on neural transmitter.