溃疡性结肠炎（UC）是现代难治病之一，目前尚无有效根治方法。最新研究表明，肠道菌群失衡和分子伴侣系统失调是UC发病的核心病理机制，而免疫和自免疫调节变化是两者交互作用的中间环节。本课题拟在前期国家自然科学基金项目研究的基础上，以治疗UC疗效好、复发少的特色蒙药嘎日迪散为研究载体，应用T-RFLP结合毛细管电泳法绘制肠道菌群指纹图谱，通过聚类分析，研究嘎日迪散对肠道菌群种属的影响；以IHC、ELISA、Western-blot及Real-time PCR技术探讨嘎日迪散对UC模型大鼠免疫与自免疫调控作用；以双免疫荧光技术观察嘎日迪散对分子伴侣系统失调的纠正作用。综合分析肠道共生菌群、免疫与自免疫调节及分子伴侣系统三者之间的关系，最终阐明嘎日迪散治疗UC的主要作用途径及效应分子靶点，从分子层面阐释蒙医治疗UC的核心治则"疏肝除浊、消粘解毒、通行赫依"的科学内涵。

Ulcerative colitis (UC) is one of the most intractable diseases at present , and there are no effective therapies currently. New research shows that, the intestinal flora imbalance and chaperone system disorder is the key mechanism of the UC pathogenesis. And the immune and autoimmune regulation change is the intermediate link between their interaction. In this study We plan to research the Mongolian Herbal Medicine GaRiDi pulvis, as it has good curative effect and less recurrence on the UC treatment based on the previous project of the National Natural Science Foundation research. To draw the fingerprint of intestinal flora , the T-RFLP combined with capillary electrophoresis is to be used and through the cluster analysis, GaRiDi pulvis is to be researched about its effect on the intestinal tract flora species; and to investigate the GaRiDi pulvis's effect on the immune and autoimmune regulation of the UC rat model, immunohistochemistry, ELISA, Western-blot and Real-time PCR Technology are to be used; and the double immunofluorescence technique is to be used for observing the GaRiDi pulvis's effect on correcting dysfunctional molecular chaperone system. We would synthetically analysis the relationship among intestinal microflora, immune and autoimmune regulation and molecular chaperone system. And the primarily functional role pathway and effective molecule target of GaRiDi pulvis in the treatment of UC is to be illustrated. The key rule of UC treatment in Mongolian medicine, " Shugan turbidity, decreasing viscosity and detoxification, and transiting Heyi", is to be explained.